Glutathione Reductase Yeast (E.coli)

Introduction to the Molecule

Glutathione reductase (GR) is a member of pyridine nucleotide-disulfide oxidoreductases, which includes the closely related enzymes thioredoxin reductase, lipoamide dehydrogenase, trypanothione reductase and mercuric ion reductase. GR is a cytoplasmic flavoenzyme widely distributed in aerobic organisms. The dimeric protein is composed of two identical subunits, each containing 1 FAD and 1 redox-active disulfide/dithiol as components of the catalytic apparatus. It plays a role in maintaining glutathione (GSH) in its reduced form by catalyzing the reduction of glutathione disulfide (GSSG):GSSG + NADPH + H+⇔2GSH + NADP+ In most eukaryotic cells, GR maintains the ratio of [GSH]/[GSSG] elevated, and participates in several vital functions such as the detoxification of reactive oxygen species as well as protein and DNA biosynthesis.

Source

E. coli

Storage, Stability/Shelf Life

Glutathione reductase is supplied with a vial of storage buffer (20mM HEPES, pH8.0 / 10% glycerol). Store at –80°C.

By molecule:

• Glutathione reductase