GM-CSF Human E. coli

Introduction to the Molecule

GM-CSF is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes. It can be produced by a wide variety of tissue types. The biological effects of GM-CSF are mediated through its binding to cell surface receptors, which appear to be widely expressed by thermatopoietic cells and also by some non-thermatopoietic cells, such as endothelial cells.

Research topic

Cytokines and chemokines and related molecules

Description

Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques.

Amino Acid Sequence

Source

E. coli

Purity

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.1 ng/ml, corresponding to a Specific Activity of 11.1×106 IU/mg.

Formulation

GM-CSF was lyophilized after extensive dialysis against 2 mM sodium phosphate buffer pH= 7.4±0.1.

Storage, Stability/Shelf Life

Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution GMCSF should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered White lyophilized powder.

By research topic:

• Cytokines and chemokines and related molecules

By molecule:

• GM-CSF