Heart Fatty Acid Binding Protein Human E. coli

Research topic

Energy metabolism and body weight regulation

Description

Total 142 AA. MW: 15.97 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

14% SDS-PAGE separation of Human FABP3 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and boiled sample, 5μg/lane 3. non-reduced and non-boiled sample, 5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for one week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

By research topic:

• Energy metabolism and body weight regulation

By molecule:

• Heart Fatty Acid Binding Protein