Heat Shock 10kDa Protein, Mitochondrial Human E. coli

Research topic

Others, Reproduction

Description

Total 111 AA. MW: 12.0 kDa (calculated). N-Terminal His-tag (10 extra AA)

Amino Acid Sequence

Source

E. coli

Purity

Purity as determined by densitometric image analysis: >90%

SDS-PAGE gel

14% SDS-PAGE separation of Human HSP10 1. M.W. marker – 97, 66, 45, 31, 21, 14 kDa 2. reduced and heated sample, 5μg/lane 3. non-reduced and non-heated sample, 5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Applications

ELISA, Western blotting

By research topic:

• Others
• Reproduction

By molecule:

• Heat Shock 10kDa Protein, Mitochondrial