HLA-G5 Human (HEK)
| Type: Recombinant | |||||
| Source: HEK293 | Species: Human | ||||
| Other names: soluble HLA-G5, HLAG isoform 5, HLA class I histocompatibility antigen alpha chain G variant 5 | Product of BioVendor | ||||
| Product: | Size: | ||||
|---|---|---|---|---|---|
| New: RD172070050 | 0.05 mg | ||||
| Files: Datasheet PDF | |||||
Product details
Research topic
Reproduction
Description
Total 306 AA. MW: 35.07 kDa (calculated). C-Terminal FLAG-tag, 11 extra AA (highlighted).
Amino Acid Sequence
GSHSMRYFSA AVSRPSRGEP RFIAMGYVDD TQFVRFDSDS ACPRMEPRAP WVEREGPEYW EEETRNTKAH AQTDRMNLQT LRGYYNQSEA SSHTLQWMIG CDLGSDGRLL RGYEQYAYDG KDYLALNEDL RSWTAADTAA QISKRKCEAA NVAEQRRAYL EGTCVEWLHR YLENGKEMLQ RADPPKTHVT HHPVFDYEAT LRCWALGFYP AEIILTWQRD GEDQTQDVEL VETKPAGDGT FQKWAAVVVP SGEEQRYTCH VQHEGLPEPL MLRWSKEGDG GIMSVRESRS LSEDLAAADY KDDDDK
Source
HEK293
SDS-PAGE gel
|
10% SDS-PAGE separation of Human HLA-G5 |
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 50mM NaCl, pH 7.5
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
Western blotting
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