Interleukin-33 Human E.coli

Description

Total 169 AA. MW: 19,2 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

14% SDS-PAGE separation of Human Interleukin-33 reduced and heated sample, 5μg / lane M.W. marker – 14, 21, 31, 45, 66, 97 kDa non-reduced and non-heated sample, 5μg / lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

By molecule:

• Interleukin-33