Interleukin-6 Human E. coli

Introduction to the Molecule

Interleukin-6 is a multi-functional cytokine that regulates immune responses, acute phase reactions and hematopoiesis, and it may play a central role in host defense mechanisms. IL-6 is usually not produced constitutively by normal cells, but its expression is readily induced by a variety of cytokines, lipopolysaccharide or viral infections.

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Description

Total 193 AA. MW: 22.05 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

14% SDS-PAGE separation of Human Interleukin-6 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg/lane 3. non-reduced and non-heated sample, 5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized pro tein remains stable until the expiry date when stored at –20°C. Aliquot reconstit uted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

References

• Hirano T, Akira S, Taga T, Kishimoto T. Biological and clinical aspects of interleukin 6. Immunol Today. 1990 Dec;11 (12):443-9
• Hirano T, Kishimoto T. Interleukin-6.Handbook of experimental pharmacology, Peptide Growth Factors and Their Receptors. Springer-Verlag. 1990;:633-665
• Hirano T, Taga T, Nakano N, Yasukawa K, Kashiwamura S, Shimizu K, Nakajima K, Pyun KH, Kishimoto T. Purification to homogeneity and characterization of human B-cell differentiation factor (BCDF or BSFp-2). Proc Natl Acad Sci U S A. 1985 Aug;82 (16):5490-4
• Potter M, Nordan RP. A macrophage-derived factor required by plasmacytomas for survival and proliferation in vitro. Science. 1986 Aug 1;233 (4763):566-9
• Ray A, Tatter SB, Santhanam U, Helfgott DC, May LT, Sehgal PB. Regulation of expression of interleukin-6. Molecular and clinical studies. Ann N Y Acad Sci. 1989;557:353-61; discussion 361-2
• Sehgal PB, Greininger G, Tosato G. Regulation of the acute phase and immune responses: interleukin-6. Ann N Y Acad Sci. 1989;557:1-583
• Van Snick J, Billiau A, Simpson RJ, Vink A, Van Damme J, Cayphas S. Identification of an interleukin HP1-like plasmacytoma growth factor produced by L cells in response to viral infection. J Immunol. 1987 Nov 1;139 (9):2965-9

By research topic:

• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• Interleukin-6