Klotho Human CHO

Research topic

Renal disease, Steroid hormones

Description

Total 522 AA. Mw: 59,5 kDa (calculated) C-terminal his-tag (6 extra AA) UniProt entry Q9UEF7–2, Isoform 2 (secreted)

Amino Acid Sequence

Source

CHO

Purity

More than 90% as determined by densitometric image analysis of coomasie stained SDS-PAGE

SDS-PAGE gel

12% SDS-PAGE separation of Human Klotho protein 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and boiled sample, 5μg/lane 3. non-reduced and non-boiled sample, 5μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.08 M phosphate buffer, 0.1 M NaCl, pH 7.2

Reconstitution

Add deionized water to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week. Avoid storage of lyophilized product at room temperature. Transport on dry ice.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL TEST to determine endotoxin level.

Applications

ELISA, Immunoassays, Western blotting

Note

This product is intended for research use only. The Certificate of Analysis is available on www.biovendor.com

By research topic:

• Renal disease
• Steroid hormones

By molecule:

• Klotho