Lecithin-cholesterol acyltransferase Human HEK293

Research topic

Lipoprotein metabolism

Description

Total 429 AA. MW: 48.5 kDa (calculated). UniProtKB/Swiss-Prot P04180 Phe25-Glu440 with N-Terminal Flag-tag, 13 extra AA (highlighted).

Amino Acid Sequence

Source

HEK293

SDS-PAGE gel

14% SDS-PAGE separation of Human LCAT 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg/lane 3. non-reduced and non-heated sample, 5μg/lane

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 20mM Tris buffer, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Western blotting

By research topic:

• Lipoprotein metabolism

By molecule:

• Lecithin-cholesterol acyltransferase