Lecithin-cholesterol acyltransferase (Phosphatidylcholine-sterol acyltransferase, LCAT) Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | LCAT, Phosphatidylcholine-sterol acyltransferase | ||
| Cat. No.: | RD172122100 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172122100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
|
Description
Total 426 AA. MW: 48.3 kDa (calculated). N-terminal His-tag, 10extra AA (highlighted).
Amino Acid Sequence
MKHHHHHHAS FWLLNVLFPP HTTPKAELSN HTRPVILVPG CLGNQLEAKL DKPDVVNWMC YRKTEDFFTI WLDLNMFLPL GVDCWIDNTR VVYNRSSGLV SNAPGVQIRV PGFGKTYSVE YLDSSKLAGY LHTLVQNLVN NGYVRDETVR AAPYDWRLEP GQQEEYYRKL AGLVEEMHAA YGKPVFLIGH SLGCLHLLYF LLRQPQAWKD RFIDGFISLG APWGGSIKPM LVLASGDNQG IPIMSSIKLK EEQRITTTSP WMFPSRMAWP EDHVFISTPS FNYTGRDFQR FFADLHFEEG WYMWLQSRDL LAGLPAPGVE VYCLYGVGLP TPRTYIYDHG FPYTDPVGVL YEDGDDTVAT RSTELCGLWQ GRQPQPVHLL PLHGIQHLNM VFSNLTLEHI NAILLGAYRQ GPPASPTASP EPPPPE
Source
E. coli
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human LCAT |
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH 4.0
Reconstitution
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
Western blotting
Note
This product is intended for research use only.
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