Matrix Metalloproteinase-13 (MMP-13) E. coli

Introduction to the Molecule

MMP-13 is a member of the matrix metralloproteinases family with broad substrate specificity and a potential role in tumor metastasis and invasion. MMP-13 was originally identified in breast carcinomas. Further studies revealed that this enzyme is produced by a variety of malignant tumors. It is a major player in the disease condition of rheumatoid arthritis and osteoarthritis.

Research topic

Others

Description

Matrix Metalloproteinase-13 Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain having a molecular mass of 27 kDa. The Collagenase 3 is purified by proprietary chromatographic techniques.

Source

E. coli

Purity

Greater than 90% as determined by SDS-PAGE

Biological Activity

Activity is determined by the cleavage of fluorogenic peptide, 100 ng of enzyme activated with APMA will digest 75–80% (1.5–1.6 nmole) of fluorogenic peptide substrate (0.1 ml of 20 µM solution) at 35°C for 30 minutes.

Formulation

The protein Solution (100 µg/ml) in 0.05 M Tris-HCl buffer, pH 7.6, containing 0.2 M NaCl, 5 mM CaCl₂, 20 µM ZnSO₄ and 0.1% BSA and NaN₃.

Storage, Stability/Shelf Life

MMP-13 although stable at 4°C for 1 week, should be stored desiccated below –18°C. Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered clear solution.

By research topic:

• Others

By molecule:

• Matrix Metalloproteinase-13 (MMP-13)