Monocyte Chemotactic Protein-1 Human E. coli

Introduction to the Molecule

Monocyte chemotactic protein-1 (MCP-1) is a member of the C-C family of chemokines. Chemokines are mediators that share the capacity to induce directional migration of leukocytes. MCP-1 is a chemoattractant for many cell types such as monocytes, neutrophils, hepatic stellate cells, granulocytes and T lymphocytes. MCP-1 is believed to play a role in a variety of pathological conditions, including inflammatory and immune reactions, atherosclerosis and neoplasia. Monocyte Chemotactic Protein-1 (MCP-1) is also known as Monocyte Chemotactic Activating Factor (MCAF) or CCL2.

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation

Description

Monocyte Chemotactic Protein-1Human Recombinant also known as Monocyte Chemotactic and Activating Factor (MCAF) produced in E.coli is a non-glycosylated, Polypeptide chain containing 76 amino acids and having a molecular mass of 8607 Dalton. The MCP-1 is purified by proprietary chromatographic techniques.

Amino Acid Sequence

Source

E. coli

Purity

Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE

Biological Activity

The specific activity as determined by the ability of MCP-1 to chemoattract human Monocytes using a concentration of 5–20 ng/ml.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing no additives.

Storage, Stability/Shelf Life

Lyophilized MCP-1 although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution CCL2 should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered White lyophilized powder.

By research topic:

• Cytokines and chemokines and related molecules
• Energy metabolism and body weight regulation

By molecule:

• Monocyte Chemotactic Protein-1