Osteoactivin/GPNMB Human HEK293

Research topic

Bone and cartilage metabolism

Description

Total 477 AA (whole extracellular domain Ala 22 – Pro 486). MW: 53.7 kDa (calculated), migrates at ~ 97 kDa on SDS-PAGE; C-terminal linker (Lys, Leu) and 10× His-tag (10 extra AA highlighted). Protein identity confirmed by MALDI-TOF MS.

Amino Acid Sequence

Source

HEK293

Purity

>95%

SDS-PAGE gel

12% SDS-PAGE separation of Human Osteoactivin M.W. marker – 14, 21, 31, 45, 66, 97 kDa reduced and boiled sample, 5μg/lane non-reduced and non-boiled sample, 5μg/lane

Endotoxin

<1.0 EU/µg

Formulation

Filtered (0.4 μm) and lyophilized in 0.5 mg/mL in 0.05 M phosphate buffer, 0.075 M NaCl, pH 7.4

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL TEST to determine quantity of endotoxin.

Applications

Cell culture and/or animal studies, ELISA, Western blotting

Note

This product is intended for research use only. The Certificate of Analysis is available on www.biovendor.com

By research topic:

• Bone and cartilage metabolism

By molecule:

• Osteoactivin/GPNMB