Pigment epithelium-derived factor (PEDF, Serpin-F1, EPC-1) Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | PEDF, Serpin-F1, EPC-1 | ||
| Cat. No.: | RD172114100 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172114100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
|
Description
Total 413 AA. MW: 46.1 kDa (calculated). N-Terminal His-tag 14 AA The (highlighted).
Amino Acid Sequence
MRGSHHHHHH GMASQNPASP PEEGSPDPDS TGALVEEEDP FFKVPVNKLA AAVSNFGYDL YRVRSSMSPT TNVLLSPLSV ATALSALSLG AEQRTESIIH RALYYDLISS PDIHGTYKEL LDTVTARQKN LKSASRIVFE KKLRIKSSFV APLEKSYGTR PRVLTGNPRL DLQEINNWVQ AQMKGKLARS TKEIPDEISI LLLGVAHFKG QWVTKFDSRK TSLEDFYLDE ERTVRVPMMS DPKAVLRYGL DSDLSCKIAQ LPLTGSMSII FFLPLKVTQN LTLIEESLTS EFIHDIDREL KTVQAVLTVP KLKLSYEGEV TKSLQEMKLQ SLFDSPDFSK ITGKPIKLTQ VEHRAGFEWN EDGAGTTPSP GLQPAHLTFP LDYHLNQPFI FVLRDTDTGA LLFIGKILDP RGP
Source
E. coli
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human PEDF |
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 150mM NaCl, pH 7.5
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
Western blotting
Note
This product is intended for research use only.
References
- Simonovic M, Gettins PG, Volz K. Crystal structure of human PEDF, a potent anti-angiogenic and neurite growth-promoting factor.
- Becerra SP, Palmer I, Kumar A, Steele F, Shiloach J, Notario V, Chader GJ. Overexpression of fetal human pigment epithelium-derived factor in Escherichia coli. A functionally active neurotrophic factor.
- Gettins PG, Simonovic M, Volz K. Pigment epithelium-derived factor (PEDF), a serpin with potent anti-angiogenic and neurite outgrowth-promoting properties.
- Becerra SP, Sagasti A, Spinella P, Notario V. Pigment epithelium-derived factor behaves like a noninhibitory serpin. Neurotrophic activity does not require the serpin reactive loop.
- Petersen SV, Valnickova Z, Enghild JJ. Pigment-epithelium-derived factor (PEDF) occurs at a physiologically relevant concentration in human blood: purification and characterization.
- Stratikos E, Alberdi E, Gettins PG, Becerra SP. Recombinant human pigment epithelium-derived factor (PEDF): characterization of PEDF overexpressed and secreted by eukaryotic cells.
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