Procalcitonin Human E. coli

Research topic

Immune Response, Infection and Inflammation, Sepsis

Description

Total 126 AA. MW: 14 037 Da (calculated). N-Terminal His-tag, 10 extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

SDS-PAGE gel

12% SDS-PAGE separation of Human Procalcitonin 1. reduced and heated sample, 5μg / lane 2. non-reduced and non-heated sample, 5μg / lane 3. M.W. marker – 14, 21, 31, 45, 66, 97 kDa

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 50mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein."

Applications

Western blotting

References

• Afsharpaiman S, Abedini M, Ashtiani MH, Khairkhah H, Zafari J, Mamishi S, Pourakbari B, Rad SS. Evaluation of procalcitonin and neopterin level in serum of patients with acute bacterial infection. Braz J Infect Dis. 2010 May-Jun;14 (3):252-5
• Determann RM, Schultz MJ. PCT and sTREM-1: the markers of infection in critically ill patients?. Med Sci Monit. 2008 Dec;14 (12):RA241-7
• Giacomuzzi F, Valent F, Falleti E, Romanello C, Miorin E, Tenore A, Pecile P. Procalcitonin: a marker of severity of acute pyelonephritis among children. Pediatrics. 2004 Aug;114 (2):e249-54
• Gurlich R, Nedelnikova K, Maruna P. Physiology and genetics of procalcitonin. Physiol Res. 2000;49 Suppl 1:S57-61
• Halevy R, Sakran W, Colodner R, Raz R, Koren A, Smolkin V. Procalcitonin as a marker of acute pyelonephritis in infants and children. Pediatr Nephrol. 2002 Jun;17 (6):409-12
• Hart CA, Thomson AP, Carrol ED. Procalcitonin as a marker of sepsis. Int J Antimicrob Agents. 2002 Jul;20 (1):1-9
• Juthani-Mehta M, Nanda N. Novel biomarkers for the diagnosis of urinary tract infection-a systematic review. Biomark Insights. 2009;4:111-21
• Lee CT, Lee JH, Yoon HI, Kwon SY, Kang YA. Role of C-reactive protein and procalcitonin in differentiation of tuberculosis from bacterial community acquired pneumonia. Korean J Intern Med. 2009 Dec;24 (4):337-42
• Mann B, Landgrafe G, Sand D, Pala XF, Bechara FG, Trullen XV, Sand M. A prospective bicenter study investigating the diagnostic value of procalcitonin in patients with acute appendicitis. Eur Surg Res. 2009;43 (3):291-7
• Okay TS, Leone C, Carcillo JA, Junior RR, Lopes MR, Troster EJ, Arkader R. Procalcitonin does discriminate between sepsis and systemic inflammatory response syndrome. Arch Dis Child. 2006 Feb;91 (2):117-20
• Shim JY, Woo HY, Park MS, Jung HL, Shim JW, Hwang SJ, Lee JY. Clinical significance of serum procalcitonin in patients with community-acquired lobar pneumonia. Korean J Lab Med. 2010 Aug;30 (4):406-13
• Vadas L, Grau GE, Ricou B, Pittet D, Froidevaux C, Holeckova K, Harbarth S, Pugin J. Diagnostic value of procalcitonin, interleukin-6, and interleukin-8 in critically ill patients admitted with suspected sepsis. Am J Respir Crit Care Med. 2001 Aug 1;164 (3):396-402

By research topic:

• Immune Response, Infection and Inflammation
• Sepsis

By molecule:

• Procalcitonin