Regenerating Protein 1 beta Human E. coli

Research topic

Diabetology - Other Relevant Products, Pancreatic regulatory molecules

Description

Total 156 AA. MW: 17.8 kDa (calculated). N-Terminal His-tag 12 AA (highlighted).

Amino Acid Sequence

Source

E. coli

Purity

>95%

SDS-PAGE gel

12% SDS-PAGE separation of Human Reg1 beta 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 2.5μg/lane 3. non-reduced and non-heated sample, 2.5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20 mM Tris , pH 8.0

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

ELISA, Western blotting

Note

This product is intended for research use only.

References

• Boonyasrisawat W, Pulsawat P, Yenchitsomanus PT, Vannasaeng S, Pramukkul P,Deerochanawong C, Sriussadaporn S, Ploybutr S, Pasurakul T, Banchuin N. Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy.
• Kinoshita Y, Ishihara S, Kadowaki Y, Fukui H, Chiba T. Reg protein is a unique growth factor of gastric mucosal cells.
• Okamoto H. The Reg gene family and Reg proteins: with special attention to the regeneration of pancreatic beta-cells.
• Tezel E, Nagasaka T, Tezel G, Kaneko T, Takasawa S, Okamoto H, Nakao A. REG I as a marker for human pancreatic acinoductular cells. Hepatogastroenterology.
• Zhang YW, Ding LS, Lai MD. Reg gene family and human diseases.

By research topic:

• Diabetology - Other Relevant Products
• Pancreatic regulatory molecules

By molecule:

• Regenerating Protein 1 beta