Regenerating Protein 1 beta (REG-1 beta) Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | REG 1 beta | ||
| Cat. No.: | RD172084100 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172084100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
|
Description
Total 156 AA. MW: 17.8 kDa (calculated). N-Terminal His-tag 12 AA (highlighted).
Amino Acid Sequence
MKHHHHHHAS HMQESQTELP NPRISCPEGT NAYRSYCYYF NEDPETWVDA DLYCQNMNSG NLVSVLTQAE GAFVASLIKE SSTDDSNVWI GLHDPKKNRR WHWSSGSLVS YKSWDTGSPS SANAGYCASL TSCSGFKKWK DESCEKKFSF VCKFKN
Source
E. coli
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human Reg1 beta |
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20 mM Tris , pH 8.0
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
ELISA, Western blotting
Note
This product is intended for research use only.
References
- Boonyasrisawat W, Pulsawat P, Yenchitsomanus PT, Vannasaeng S, Pramukkul P,Deerochanawong C, Sriussadaporn S, Ploybutr S, Pasurakul T, Banchuin N . Analysis of the reg1alpha and reg1beta gene transcripts in patients with fibrocalculous pancreatopathy.
- Zhang YW, Ding LS, Lai MD. Reg gene family and human diseases.
- Tezel E, Nagasaka T, Tezel G, Kaneko T, Takasawa S, Okamoto H, Nakao A. REG I as a marker for human pancreatic acinoductular cells. Hepatogastroenterology.
- Kinoshita Y, Ishihara S, Kadowaki Y, Fukui H, Chiba T. Reg protein is a unique growth factor of gastric mucosal cells.
- Okamoto H. The Reg gene family and Reg proteins: with special attention to the regeneration of pancreatic beta-cells.
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