Retinol binding protein 4 (RBP4) Human (E. coli)

Description

Total 204 AA. MW: 23 kDa (calculated). N-terminal His-tag, 21 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q5VY30 (AA16–199).

Amino Acid Sequence

Source

E. coli

Purity

95%, as determined by SDS-PAGE.

SDS-PAGE gel

12% SDS-PAGE separation of Human RBP4 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg / lane 3. non-reduced and non-heated sample, 5μg / lane

Biological Activity

Determined by its ability to bind all trans retinol acids. The binding of RBP4 to all trans retinol acids results in quenching of Trp fluroscence. 0.5 M retinol acids/per M RBP4.

Endotoxin

<0.5 EU/ug protein.

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in PBS buffer, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Binding assay, ELISA, Western blotting

Note

This product is intended for research use only.