S100Z Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | Tagless | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Cat. No.: | RD172049100 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172049100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
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Description
Total 99 AA. MW: 11.5 kDa (calculated). One N-Terminal extra AA (highlighted).
Amino Acid Sequence
MPTQLEMAMD TMIRIFHRYS GKARKRFKLS KGELKLLLQR ELTEFLSCQK ETQLVDKIVQ DLDANKDNEV DFNEFVVMVA ALTVACNDYF VEQLKKKGK
Source
E. coli
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human S100Z |
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05M Phosphate buffer, 0.1M NaCl, pH7.2
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Quality Control Test
BCA – to determine content of the protein
SDS PAGE – to determine purity of the protein
Applications
ELISA, Western blotting
Introduction to the Molecule
S100Z is a recently discovered member of the S100 protein family. S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner.
S100Z is a 99-amino acid protein capable of interacting with another member of the family, S100P.
There are differences in the expression level of S100Z mRNA in various tissues. The highest levels were found in spleen and leukocytes. S100Z gene expression appears to be deregulated in some tumor tissues, compared to expression in their normal counterparts.
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