S100Z Human (E. coli)

Description

Total 99 AA. MW: 11.5 kDa (calculated). One N-Terminal extra AA (highlighted).

Amino Acid Sequence

Source

E. coli

Purity

>95%

SDS-PAGE gel

12% SDS-PAGE separation of Human S100Z 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg/lane 3. non-reduced and non-heated sample, 5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.05M Phosphate buffer, 0.1M NaCl, pH7.2

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Quality Control Test

BCA – to determine content of the protein

SDS PAGE – to determine purity of the protein

Applications

ELISA, Western blotting

Introduction to the Molecule

S100Z is a recently discovered member of the S100 protein family. S100 proteins are small dimeric members of the EF-hand superfamily of Ca(2+) binding proteins thought to participate in mediating intracellular Ca(2+) signals by binding to and thereby regulating target proteins in a Ca(2+)-dependent manner.

S100Z is a 99-amino acid protein capable of interacting with another member of the family, S100P.

There are differences in the expression level of S100Z mRNA in various tissues. The highest levels were found in spleen and leukocytes. S100Z gene expression appears to be deregulated in some tumor tissues, compared to expression in their normal counterparts.