soluble Advanced glycation end product-specific receptor (sRAGE) Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | sRAGE, RAGE | ||
| Cat. No.: | RD172116100 | ||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172116100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: |  new product discount |
 temporarily out of stock order in advance |
|
Description
Total 339 AA. MW: 36.5 kDa. N-Terminal His-tag 14 AA (highlighted).
Amino Acid Sequence
MRGSHHHHHH GMASAQNITA RIGEPLVLKC KGAPKKPPQR LEWKLNTGRT EAWKVLSPQG GGPWDSVARV LPNGSLFLPA VGIQDEGIFR CQAMNRNGKE TKSNYRVRVY QIPGKPEIVD SASELTAGVP NKVGTCVSEG SYPAGTLSWH LDGKPLVPNE KGVSVKEQTR RHPETGLFTL QSELMVTPAR GGDPRPTFSC SFSPGLPRHR ALRTAPIQPR VWEPVPLEEV QLVVEPEGGA VAPGGTVTLT CEVPAQPSPQ IHWMKDGVPL PLPPSPVLIL PEIGPQDQGT YSCVATHSSH GPQESRAVSI SIIEPGEEGP TAGEGFDKVR EAEDSPQHM
Source
E. coli
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human sRAGE |
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 0.03 M Acetate buffer, pH 4
Reconstitution
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
Western blotting
Note
This product is intended for research use only.
Introduction to the Molecule
The receptor for advanced glycation end products (RAGE) is a multiligand member of the immunoglobulin superfamily of cell surface molecules. It mediates interactions of advanced glycation end products (AGE), the products of nonenzymatic glycoxidation of proteins/lipids that accumulate in the plasma and tissues of patients with diabetes.
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