soluble Advanced glycation end product-specific receptor (sRAGE) Human (HEK)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | Flag | ||
| Source: | HEK293 | ||
| Species: | Human | ||
| Other names: | sRAGE, RAGE | ||
| Cat. No.: |
RD172116100-HEK |
||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172116100-HEK+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF | ||
| Legend: | new product discount |
temporarily out of stock order in advance |
|
Description
Total 331 AA, MW: 35.2 kDa (calculated). C-terminal Flag-tag 11 AA (highlighted).
Amino Acid Sequence
AQNITARIGE PLVLKCKGAP KKPPQRLEWK LNTGRTEAWK VLSPQGGGPW DSVARVLPNG SLFLPAVGIQ DEGIFRCQAM NRNGKETKSN YRVRVYQIPG KPEIVDSASE LTAGVPNKVG TCVSEGSYPA GTLSWHLDGK PLVPNEKGVS VKEQTRRHPE TGLFTLQSEL MVTPARGGDP RPTFSCSFSP GLPRHRALRT APIQPRVWEP VPLEEVQLVV EPEGGAVAPG GTVTLTCEVP AQPSPQIHWM KDGVPLPLPP SPVLILPEIG PQDQGTYSCV ATHSSHGPQE SRAVSISIIE PGEEGPTAGS VGGSGLGTLA AAADYKDDDD K
Source
HEK293
Purity
>95%
SDS-PAGE gel
|
12% SDS-PAGE separation of Human sRAGE |
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/mL in 50 mM Tris-HCl pH 7.5, 50 mM NaCl.
Reconstitution
Add deionized water to a working concentration approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter this product by appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at ‑20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. The lyophilized protein remains stable until the expiry date when stored at ‑20°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
ELISA, Western blotting
Note
This product is intended for research use only.
Introduction to the Molecule
The receptor for advanced glycation end products (RAGE) is a multiligand member of the immunoglobulin superfamily of cell surface molecules. It mediates interactions of advanced glycation end products (AGE), the products of nonenzymatic glycoxidation of proteins/lipids that accumulate in the plasma and tissues of patients with diabetes.
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RD172116100-HEK
discount
temporarily out of stock
order in advance