Stanniocalcin 2 (STC-2) Human (HEK)

Description

Total 289 AA. Mw: 31.9 kDa (calculated). C-Terminal Flag-tag, 12AA (highlighted). The AA sequence is identical to Swiss-Prot- O76061 (STC2, aa 25–302).

Amino Acid Sequence

Source

HEK293

Purity

>90%

SDS-PAGE gel

12% SDS-PAGE separation of Human STC-2 1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5μg/lane 3. non-reduced and non-heated sample, 5μg/lane

Formulation

Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM Tris buffer, 20 mM NaCl, pH 7.5

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Storage, Stability/Shelf Life

Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

Applications

Cell culture and/or animal studies, ELISA, Western blotting

Note

This product is intended for research use only.

Introduction to the Molecule

Stanniocalcin-2 is a secreted protein, homodimer, disulfide-linked. It has an anti-hypocalcemic action on calcium and phosphate homeostasis. It is expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leucocytes. It belongs to the stanniocalcin family.

References

• Mittapalli VR, Prols F, Huang R, Christ B, Scaal M. Avian stanniocalcin-2 is expressed in developing striated muscle and joints.
• Ito D, Walker JR, Thompson CS, Moroz I, Lin W, Veselits ML, Hakim AM, Fienberg AA, Thinakaran G. Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties.
• Paciga M, James K, Gillespie JR, Wagner GF. Evidence for cross-talk between stanniocalcins.
• Gagliardi AD, Kuo EY, Raulic S, Wagner GF, DiMattia GE. Human stanniocalcin-2 exhibits potent growth-suppressive properties in transgenic mice independently of growth hormone and IGFs.
• Esseghir S, Kennedy A, Seedhar P, Nerurkar A, Poulsom R, Reis-Filho JS, Isacke CM. Identification of NTN4, TRA1, and STC2 as prognostic markers in breast cancer in a screen for signal sequence encoding proteins.
• Yahata K, Mori K, Mukoyama M, Sugawara A, Suganami T, Makino H, Nagae T, Fujinaga Y, Nabeshima Y, Nakao K. Regulation of stanniocalcin 1 and 2 expression in the kidney by klotho gene.
• Jellinek DA, Chang AC, Larsen MR, Wang X, Robinson PJ, Reddel RR. Stanniocalcin 1 and 2 are secreted as phosphoproteins from human fibrosarcoma cells.
• Moore EE, Kuestner RE, Conklin DC, Whitmore TE, Downey W, Buddle MM, Adams RL, Bell LA, Thompson DL, Wolf A, Chen L, Stamm MR, Grant FJ, Lok S, Ren H, De Jongh KS. Stanniocalcin 2: characterization of the protein and its localization to human pancreatic alpha cells.