Trefoil factor 2 (Spasmolytic polypeptide, Spasmolysin , SP, TFF2) Human (E.coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | Spasmolytic polypeptide, SP, Spasmolysin, TFF2 | ||
| Cat. No.: |
 RD172159100 |
||
| Size: | 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
|
Description
Total 116 AA. MW: 13,2 kDa (calculated). N-Terminal His-tag, 10 extra AA (highlighted).
Amino Acid Sequence
MKHHHHHHAS EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
Source
E. coli
Purity
>95%
Formulation
Filtered (0,4 μm) and lyophilized in 0.5 mg/mL in 20mM TRIS, 20mM NaCl, pH 7.5
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
ELISA, Western blotting
Note
This product is intended for research use only.
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