Vaspin (Serpin A12, Visceral adipose-specific serpin) Human (E. coli)
| Type: | Recombinant | ||
|---|---|---|---|
| Tag: | His | ||
| Source: | E. coli | ||
| Species: | Human | ||
| Other names: | Visceral adipose-specific serpin, Serpin A12 | ||
| Cat. No.: |
 RD172097100 |
||
| Size: | 0.1 mg | | | |
| Cat. No.: | RD172097100+ | ||
| Size: | 10 x 0.1 mg | | | |
| Files: | Datasheet PDF MSDS | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
|
Description
Total 408 AA. MW: 46.7 kDa (calculated). N-terminal His-tag, 14 extra AA (highlighted). The AA sequence is identical to UniProtKB/Swiss-Prot entry Q8IW75.
Amino Acid Sequence
MRGSHHHHHH GMASLKPSFS PRNYKALSEV QGWKQRMAAK ELARQNMDLG FKLLKKLAFY NPGRNIFLSP LSISTAFSML CLGAQDSTLD EIKQGFNFRK MPEKDLHEGF HYIIHELTQK TQDLKLSIGN TLFIDQRLQP QRKFLEDAKN FYSAETILTN FQNLEMAQKQ INDFISQKTH GKINNLIENI DPGTVMLLAN YIFFRARWKH EFDPNVTKEE DFFLEKNSSV KVPMMFRSGI YQVGYDDKLS CTILEIPYQK NITAIFILPD EGKLKHLEKG LQVDTFSRWK TLLSRRVVDV SVPRLHMTGT FDLKKTLSYI GVSKIFEEHG DLTKIAPHRS LKVGEAVHKA ELKMDERGTE GAAGTGAQTL PMETPLVVKI DKPYLLLIYS EKIPSVLFLG KIVNPIGK
Source
E. coli
SDS-PAGE gel
|
12% SDS-PAGE separation of Human Vaspin |
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml in 0.05M Acetate buffer, pH 4
Reconstitution
Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10μg/mL. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Storage, Stability/Shelf Life
Store lyophilized protein at –20°C. Lyophilized protein remains stable until the expiry date when stored at –20°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
Applications
Western blotting
Note
This product is intended for research use only.
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