Endotoxin removal

EndoTrap®, affinity chromatography for highly efficient endotoxin removal, manufactured by Hyglos GmbH.

EndoTrap® reliably removes endotoxins (lipopolysaccha­rides / LPS) from aqueous solutions of e.g. proteins, antibodies, vaccines, nucleic acids, buffers and various other substances with excellent sample recovery rates.

Features:

• Highly specific and efficient LPS removal from complex mixtures
• Quick and easy to use in column (gravity flow) or batch mode
• Upscaling on fully automated liquid-chromatographic systems possible
• Average sample/protein recovery > 95%
• Broad pH tolerance and broad pI range of target proteins
• Compatible with commonly used buffers and high salt conditions
• Reusable at least 10 times (EndoTrap® HD)
• Not Polymyxin B based, non toxic

The EndoTrap® product family consists of three members:

EndoTrap® blue
with a broad pH and salt tolerance, works best with calcium or magnesium compatible buffers such as TRIS, HEPES, MOPS but also PBS, if enriched freshly with 0.1 mM Ca/Mg2+.

EndoTrap® red
especially for the use with PBS based samples.

EndoTrap® HD
is a further development of EndoTrap EndoTrap® blue for challenging samples and large scale endotoxin removal in e.g biopharmaceutical production processes. For EndoTrap® HD additionally is available the EndoTrap® leakage ELISA.