Amino Acid Sequence
determined by its ability to stimulate chick E9 DRG neurite outgrowth. The ED50 is 1.0 ng/ml, corresponding to a specific activity of 1×106 units/mg.
Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.