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8-OH-dG StressXpress® ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:8-Hydroxy-2'-deoxyguanosine, 2'-Deoxy-7,8-dihydro-8-oxoguanosine
  • Species:Multispecies
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Cat. No. Size Price


RSKT-120-96R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Urine, Plasma

Storage/Expiration

 – 20°C

Calibration Range

100–30 pg/mL

Limit of Detection

30 pg/mL

Summary

Features

  • ELISA kit used to quantitate Hsp65 concentration in samples
  • The total assay time is less than 24 hours

Research topic

Animal studies, Oxidative stress

Summary

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).

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