| Cat. No. | Size |
Price |
|
|---|---|---|---|
| SKT-120-96 | 96 wells (1 kit) |
Cat # changed from RSKT-120-96R to SKT-120-96
Competitive ELISA, Immobilized antibody
Urine, Plasma
– 20°C
100–30 pg/mL
30 pg/mL
Oxidative stress, Animal studies
8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).