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Distributed product

8-OHdG Check (Ultrasensitive) Human ELISA (Multispecies specificity)

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:8-Hydroxy-2'-deoxyguanosine, 2'-Deoxy-7,8-dihydro-8-oxoguanosine
  • Species:Multispecies
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Cat. No. Size Price


RSCN213101R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Urine, Tissue extract

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

All reagents should be stored at 2–8°C. The kit should not be used beyond 9 months past the manufacturers date stamped on the exterior of the box.

Calibration Curve

Calibration Range

0.125 –10 ng/ml

Summary

Research topic

Oxidative stress, Animal studies

Summary

8-OHdG is a widely used biomarker for the measurement of endogenous oxidative DNA damage.

References to Product

References

  • H.Kasai, P.F.Crain, Y.Kuchino, S.Nishimura, A.Ootsuyama, and H.Tanooka  Carcinogenesis 7, 1849­1851 (1986)  H.Ochi, M.Hashimoto, J.Kurashige  Assessment of functional tea in human using oxidative stress profile  technique. Proceedings of 2001 International Conference on O­Cha (tea) Culture and Science  (2001) 
  • M.D.Evans, M.S.Cooke, I.D.Podmore, Q.Zheng, K.E.Herbert, and J.Lunec  Discrepancies in the measurement of  UVC­induced 8­oxo­2'­deoxyguanosine: Implications for the analysis of oxidative DNA damage. Biochemical and  Biophysical Research Communications 259 pp374­378 (1999) 
  • M.S.Cooke, M.D.Evans and J.Lunec  DNA repair: insights from urinary lesion analysis. Free Radical Research  36(9) pp929­932 (2002) 
  • S.Okamoto, and H.Ochi  Chemical Abst. 129859a (1992)
  • S.Toyokuni, T.Tanaka, Y.Hattori, Y.Nishiyama, A.Yoshida, K.Uchida, H.Hiai, H.Ochi, and T.Osawa  Lab.Invest.  76, 365­374 (1997) 
  • Tadashi Matsubasa, Takako Uchino, Shinnyo Karashima, Yuichi Kondo, Kenichi Maruyama, Masako Tanimura  and Fumio Endo  Oxidative Stress in very low Birth Weight Infants As Measured by Urinary 8­OHdG.  Free  Radical Research 36(2) pp189­193 (2002) 
  • Tomoko Shimoike, Toyoshi Inoguchi, Fumio Umeda, Hajime Nawata, Katsumi Kawano and Hirotomo Ochi  The meaning of serum levels of advanced glycosylation end products in diabetic nephropathy.  Metabolism  49(8) pp1030­1035 (2000) 
  • WenYing Fan, Kazunori Ogusu, Katsuyasu Kouda, Harunobu Nakamura, Tomoaki Satoh, Hirotomo Ochi and  Hiroichi Takeuchi  Reduced oxidative DNA damage by vegetable juice intake: A controlled trial.  J Physiol  Anthropol 19(6) pp287­289 (2000) 
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