Type
Competitive ELISA
Applications
Serum, Urine, Plasma
Sample Requirements
1 ml/ plasma and serum sample
25 µl urine sample
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
Short immunological reaction: 0,19-25 nM
Long immunological reaction: 0,09-12,5 nM
Research topic
Animal studies
Summary
AcSDKP is a new reliability marker of chronic ACE inhibition. The tetrapeptide N-acetyl-seryl-aspartyl-lysyl-proline (AcSDKP) is an endogenous regulatory factor of hematopoiesis which reverses stem cells and normal early progenitors into S-phase. Angiotensin I-Converting Enzyme (ACE) has two homologous active NH2– and COOH-terminal domains and displays activity toward a broad range of substrates. The AcSDKP has been shown to be hydrolyzed by ACE and to be a preferential substrate for its NH2-terminal active site. In healthy subjects, the acute administration of the ACE inhibitor captopril increases the AcSDKP plasma levels. Several studies aimed to measure plasma or urine AcSDKP levels during treatment with various ACE inhibitors and to confirm its relevance as a marker of ACE inhibition.