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Advantages

Retriever preserves tissue morphology

Due to the properly adjusted pressure/heating cycle the Retriever does not allow boiling of the buffer in chambers, thus preventing formation of the air bubbles that affect tissue morphology. On the images below we show unprocessed intestinal tissue, tissue processed in microwave (the standard result) and tissue processed in the Retriever. Please note that even the mucus produced by intestinal cells remains fully unaffected and the cells have the proper morphology, with the shape of the cell walls and of the nucleus exactly the same as in unprocessed section.

6702

This is a section of intestinal tissue fixed with formalin and embedded into paraffin.

6701

Processing the tissue in microwave-type machines results in damage of the morphology of the delicate tissues.

6700

No damage to morphology occurs, however, after processing the tissue in Retriever, although the unmasking is usually better than after the microwave.

Excellent Staining and Excellent Tissue Morphology

Unaffected morphology of tissues processed in Retriever does not mean poor processing. As you can see on the images below cell surface antigens, as well as nuclear ones are well detected by antibodies. On the left you can see a test staining of cervix tissue with anti-E-cadherin antibody, below are stainings for CD8, Ki67 and PCNA. Different buffers were used for each (citrate, acid citrate or EDTA). Please note the preservation of morphology and the intensity of staining without background.

6699

​CD8 (mAb)

Infiltrating cytotoxic CD8+ T- cells were detected in formalin fixed, paraffin embedded section of human intestine after antigen unmasking in Retriever. Buffer B was used for processing.

6698

​PCNA (mAb)

Marker for proliferating cells is shown for cells of human intestine. Note the morphology of the tissue that is completely unchanged by processing.

6697

Ki-67 (antibody MIB1)

Routine proliferation marker detected on formalin-fixed paraffin embedded section of human intestine. Please note the strong nuclear staining, extremely clean background (no non-specific binding of the antibody) and excellent morphology of the tissue including the preservation of the mucosal secret in the cells and undamaged membranes. Buffer A was used for processing.

Product Size Price
1.5mm

Type: Puncher Tip

TAT06-1.5 1 ea
 
1.5mm (10 x 15 ; 150 wells)

Type: Mold Kit

TAM01-1.5 1 ea
 
1.5mm (9 x 10 ; 90 wells)

Type: Premade Recipient Block

TAB06-1.5 1 ea
 
1mm

Type: Puncher Tip

TAT06-1 1 ea
 
1mm (10 x 12 ; 120 wells)

Type: Premade Recipient Block

TAB06-1 1 ea
 
1mm (10 x 17 ; 170 wells)

Type: Mold Kit

TAM01-1 1 ea
 
2mm

Type: Puncher Tip

TAT06-2 1 ea
 
2mm (6 x 10 ; 60 wells)

Type: Premade Recipient Block

TAB06-2 1 ea
 
2mm (7x 10 ; 70 wells)

Type: Mold Kit

TAM01-2 1 ea
 
3mm

Type: Puncher Tip

TAT06-3 1 ea
 
3mm (5 x 6 ; 30 wells)

Type: Premade Recipient Block

TAB06-3 1 ea
 
3mm (5 x 8 ; 40 wells)

Type: Mold Kit

TAB01-3 1 ea
 
5mm

Type: Puncher Tip

TAT06-5 1 ea
 
5mm (3 x 5 ; 15 wells)

Type: Mold Kit

TAB-01-5 1 ea
 
5mm (4 x 5 ; 20 wells)

Type: Premade Recipient Block

TAB06-5 1 ea
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-050 50 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-125 125 ml
 
Antibody Diluent For Frozen Sections ready-to-use

Type: IHC Buffers

AP0475-500 500 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0742-050 50 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-125 125 ml
 
Antibody Diluent ready-to-use

Type: IHC Buffers

AP0472-500 500 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-050 50 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-125 125 ml
 
Antibody Diluent W ready-to-use

Type: Western Blot Buffers

AP0501-500 500 ml
 
Base mold

Type: Mold Kit

TABM06 1 ea
 
Basket

Type: Accessories

R21008 1 each
 
Basket Lid for Chambers

Type: Accessories

R21009 1 each
 
Coating Buffer 7.4

Type: ELISA

AP0511-500 500 ml
 
Coating Buffer 9.6

Type: ELISA

AP0510-500 500 ml
 
Cord Set EU

Type: Accessories

R21005 1 each
 
Embedding cassette

Type: Mold Kit

TAC06 500/PK
 
Full set with 5 Tips and 5 sized blocks

Type: Tissue Microarrayer Set

TAT06 1 set
 
Green Silicone Sealing Gasket

Type: Accessories

R21007 1 each
 
Guide for 1mm recipient block

Type: Puncher Tip

TAG06 1 set
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-050W 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-125W 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: Western Blot Buffers

AP0473-500W 500 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-050E 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-125E 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: ELISA

AP0473-500E 500 m
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-050 50 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-125 125 ml
 
Peroxidase Conjugate Diluent ready-to-use

Type: IHC Buffers

AP0473-500 500 ml
 
Plate Block E

Type: ELISA

AP0513-500 500 ml
 
Plate Block, No Components of Animal Origin

Type: ELISA

AP0512-125 125 ml
 
Retriever Unit

Type: Retriever Unit

R2100-EU 1 each
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-050 50 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-125 125 ml
 
Sample & Antibody Diluent E ready-to-use

Type: ELISA

AP0514-500 500 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-050 50 ml
 
Section Block ready-to-use

Type: IHC Buffers

AP0471-125 125 ml
 
Single set for 1 mm puncher with a guide

Type: Tissue Microarrayer Set

TAT06T1 1 set
 
Single set for 1.5mm puncher

Type: Tissue Microarrayer Set

TAT06T1.5 1 set
 
Single set for 2mm puncher

Type: Tissue Microarrayer Set

TAT06T2 1 set
 
Single set for 3mm puncher

Type: Tissue Microarrayer Set

TAT06T3 1 set
 
Single set for 5mm puncher

Type: Tissue Microarrayer Set

TAT06T5 1 set
 
Slide Chamber

Type: Accessories

R21001 Set of 3
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-050 50 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-125 125 ml
 
Stripping Buffer ready-to-use W

Type: Western Blot Buffers

AP0503-500 500 ml
 
Universal Buffer

Type: Universal Buffers

AP0530-125 125 ml, 10x
 
Universal Buffer

Type: Universal Buffers

AP0530-500 500 ml, 10x
 
Washing Buffer E PBS-Based 10x

Type: ELISA

AP0515-500 500 ml
 
Washing Buffer W Tris-Based 10x

Type: Western Blot Buffers

AP0502-500 500 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-125 125 ml
 
Western Block, BSA-Free ready-to-use

Type: Western Blot Buffers

AP0500-500 500 ml