Retriever preserves tissue morphology
Due to the properly adjusted pressure/heating cycle the Retriever does not allow boiling of the buffer in chambers, thus preventing formation of the air bubbles that affect tissue morphology. On the images below we show unprocessed intestinal tissue, tissue processed in microwave (the standard result) and tissue processed in the Retriever. Please note that even the mucus produced by intestinal cells remains fully unaffected and the cells have the proper morphology, with the shape of the cell walls and of the nucleus exactly the same as in unprocessed section.
This is a section of intestinal tissue fixed with formalin and embedded into paraffin.
Processing the tissue in microwave-type machines results in damage of the morphology of the delicate tissues.
No damage to morphology occurs, however, after processing the tissue in Retriever, although the unmasking is usually better than after the microwave.
Excellent Staining and Excellent Tissue Morphology
Unaffected morphology of tissues processed in Retriever does not mean poor processing. As you can see on the images below cell surface antigens, as well as nuclear ones are well detected by antibodies. On the left you can see a test staining of cervix tissue with anti-E-cadherin antibody, below are stainings for CD8, Ki67 and PCNA. Different buffers were used for each (citrate, acid citrate or EDTA). Please note the preservation of morphology and the intensity of staining without background.
Infiltrating cytotoxic CD8+ T- cells were detected in formalin fixed, paraffin embedded section of human intestine after antigen unmasking in Retriever. Buffer B was used for processing.
Marker for proliferating cells is shown for cells of human intestine. Note the morphology of the tissue that is completely unchanged by processing.
Ki-67 (antibody MIB1)
Routine proliferation marker detected on formalin-fixed paraffin embedded section of human intestine. Please note the strong nuclear staining, extremely clean background (no non-specific binding of the antibody) and excellent morphology of the tissue including the preservation of the mucosal secret in the cells and undamaged membranes. Buffer A was used for processing.