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Manufactured by BioVendor

Afamin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:AFM, α-1T-glycoprotein
  • Species:Human
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Cat. No. Size Price

Availability on Request RD194428100R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


Serum, Plasma

Sample Requirements

5 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.15–1.6 µg/ml

Limit of Detection

0.022 µg/ml

Intra-assay (Within-Run)

n = 8; 3.0 %

Inter-assay (Run-to-Run)

n = 5; 2.8 %

Spiking Recovery

100.5 %

Dilution Linearity

100.1 %



  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures total afamin in human serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is a serum based protein
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation


Mature human afamin, the product of the AFM gene, is a single chain 75 kDa protein consisting of 578 amino acid residues. It contains three consecutive albumin domains (aa 36–206, 211–403 and 404–599) that contain characteristic 5 or 6 intra-chain disulfide bonds. AFM is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally related serum transport proteins that are known to be evolutionarily related. The glycoprotein afamin is located on chromosome 4q11–q13 in humans. Afamin is predominantly expressed in the liver and secreted into the bloodstream; minor expressions have been described also in human brain [2], heart, kidney, testis and ovary. Afamin has been reported to bind vitamin E, especially α-tocopherol and γ-tocopherol, two of the most important forms of vitamin E, in vitro and in vivo and to possess multiple binding sites for both tocopherol isomers. Comparative proteomics has previously identified afamin as a potential biomarker for ovarian cancer. Patients with ovarian cancer displayed significantly decreased plasma concentrations of afamin by comparison to healthy controls and, very recently, extended by showing significant associations between afamin plasma concentrations and clinical outcomes (response to therapy and survival rates) were reported. Human plasma afamin is reported to be strongly associated with criteria for metabolic syndrome. Data from a prospective study design as well as corresponding data from afamin-transgenic mice suggest an active role of afamin in the development of metabolic syndrome [7]. In patients with polycystic ovary syndrome, afamin might serve as a discriminatory predictive parameter of insulin resistance and metabolic syndrome.

Product References (5)


  • Kurdiova T, Balaz M, Kovanicova Z, Zemkova E, Kuzma M, Belan V, Payer J, Gasperikova D, Dieplinger H, Ukropcova B, Ukropec J. Serum Afamin a Novel Marker of Increased Hepatic Lipid Content. Front Endocrinol (Lausanne). 2021 Sep 16;12:670425. doi: 10.3389/fendo.2021.670425. eCollection 2021. PubMed PMID: 34603196. PubMed CentralPMCID: PMC8481912. See more on PubMed
  • Köninger A, Enekwe A, Mach P, Andrikos D, Schmidt B, Frank M, Birdir C, Kimmig R, Gellhaus A, Dieplinger H. Afamin: an early predictor of preeclampsia. Arch Gynecol Obstet. 2018 Nov;298(5):1009-1016. doi: 10.1007/s00404-018-4897-z. Epub 2018 Sep 15. PubMed PMID: 30220025. PubMed CentralPMCID: PMC6182689. See more on PubMed
  • Köninger A, Iannaccone A, Hajder E, Frank M, Schmidt B, Schleussner E, Kimmig R, Gellhaus A, Dieplinger H. Afamin predicts gestational diabetes in polycystic ovary syndrome patients preconceptionally. Endocr Connect. 2019 May 1;8(5):616-624. doi: 10.1530/EC-19-0064. PubMed PMID: 30991357. PubMed CentralPMCID: PMC6510713. See more on PubMed
  • Köninger A, Mathan A, Mach P, Frank M, Schmidt B, Schleussner E, Kimmig R, Gellhaus A, Dieplinger H. Is Afamin a novel biomarker for gestational diabetes mellitus? A pilot study. Reprod Biol Endocrinol. 2018 Mar 27;16(1):30. doi: 10.1186/s12958-018-0338-x. PubMed PMID: 29587878. PubMed CentralPMCID: PMC5870691. See more on PubMed
  • Dieplinger H, Dieplinger B. Afamin--A pleiotropic glycoprotein involved in various disease states. Clin Chim Acta. 2015 Jun 15;446:105-10. doi: 10.1016/j.cca.2015.04.010. Epub 2015 Apr 16. PubMed PMID: 25892677. See more on PubMed
Summary References (7)

References to Afamin

  • Jackson D, Craven RA, Hutson RC, Graze I, Lueth P, Tonge RP, Hartley JL,Nickson JA, Rayner SJ, Johnston C, Dieplinger B, Hubalek M, Wilkinson N, PerrenTJ, Kehoe S, Hall GD, Daxenbichler G, Dieplinger H, Selby PJ, Banks RE. Proteomicprofiling identifies afamin as a potential biomarker for ovarian cancer. ClinCancer Res. 2007 Dec 15;13(24):7370-9. PubMed PMID: 18094419. See more on PubMed
  • Jerkovic L, Voegele AF, Chwatal S, Kronenberg F, Radcliffe CM, Wormald MR,Lobentanz EM, Ezeh B, Eller P, Dejori N, Dieplinger B, Lottspeich F, Sattler W,Uhr M, Mechtler K, Dwek RA, Rudd PM, Baier G, Dieplinger H. Afamin is a novelhuman vitamin E-binding glycoprotein characterization and in vitro expression. J Proteome Res. 2005 May-Jun;4(3):889-99. PubMed PMID: 15952736. See more on PubMed
  • Kim BJ, Lee YS, Lee SY, Park SY, Dieplinger H, Ryu SH, Yea K, Choi S, Lee SH, Koh JM, Kim GS. Afamin secreted from nonresorbing osteoclasts acts as a chemokinefor preosteoblasts via the Akt-signaling pathway. Bone. 2012 Sep;51(3):431-40.doi: 10.1016/j.bone.2012.06.015. Epub 2012 Jun 27. PubMed PMID: 22749887. See more on PubMed
  • Kronenberg F, Kollerits B, Kiechl S, Lamina C, Kedenko L, Meisinger C, WilleitJ, Huth C, Wietzorrek G, Altmann ME, Thorand B, Melmer A, Dähnhardt D, Santer P, Rathmann W, Paulweber B, Koenig W, Peters A, Adham IM, Dieplinger H. Plasmaconcentrations of afamin are associated with the prevalence and development ofmetabolic syndrome. Circ Cardiovasc Genet. 2014 Dec;7(6):822-9. doi:10.1161/CIRCGENETICS.113.000654. Epub 2014 Aug 31. PubMed PMID: 25176938. See more on PubMed
  • Melmer A, Fineder L, Lamina C, Kollerits B, Dieplinger B, Braicu I, Sehouli J,Cadron I, Vergote I, Mahner S, Zeimet AG, Castillo-Tong DC, Ebenbichler CF,Zeillinger R, Dieplinger H. Plasma concentrations of the vitamin E-bindingprotein afamin are associated with overall and progression-free survival andplatinum sensitivity in serous ovarian cancer--a study by the OVCAD consortium.Gynecol Oncol. 2013 Jan;128(1):38-43. doi: 10.1016/j.ygyno.2012.09.032. Epub 2012Oct 9. PubMed PMID: 23063758. See more on PubMed
  • Seeber B, Morandell E, Lunger F, Wildt L, Dieplinger H. Afamin serumconcentrations are associated with insulin resistance and metabolic syndrome inpolycystic ovary syndrome. Reprod Biol Endocrinol. 2014 Sep 10;12:88. doi:10.1186/1477-7827-12-88. PubMed PMID: 25208973; PubMed Central PMCID: PMC4171562. See more on PubMed
  • Seeber BE, Czech T, Buchner H, Barnhart KT, Seger C, Daxenbichler G, Wildt L, Dieplinger H. The vitamin E-binding protein afamin is altered significantly inthe peritoneal fluid of women with endometriosis. Fertil Steril. 2010Dec;94(7):2923-6. doi: 10.1016/j.fertnstert.2010.05.008. Epub 2010 Jun 17. PubMedPMID: 20858448; PubMed Central PMCID: PMC2996608. See more on PubMed
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