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Manufactured by BioVendor

Afamin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:AFM, α-1T-glycoprotein
  • Species:Human
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Cat. No. Size Price


RD194428100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma (EDTA, citrate, heparin)

Sample Requirements

5 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.15–1.6 µg/ml

Limit of Detection

0.022 µg/ml

Intra-assay (Within-Run)

n = 8; 3.0 %

Inter-assay (Run-to-Run)

n = 5; 2.8 %

Spiking Recovery

100.5 %

Dilutation Linearity

100.1 %

Summary

Features

  • It is intended for research use only
  • The total assay time is less than 3 hours
  • The kit measures total afamin in human serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Standard is a serum based protein
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

Mature human afamin, the product of the AFM gene, is a single chain 75 kDa protein consisting of 578 amino acid residues. It contains three consecutive albumin domains (aa 36–206, 211–403 and 404–599) that contain characteristic 5 or 6 intra-chain disulfide bonds. AFM is a member of the albumin gene family, which is comprised of four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally related serum transport proteins that are known to be evolutionarily related. The glycoprotein afamin is located on chromosome 4q11–q13 in humans. Afamin is predominantly expressed in the liver and secreted into the bloodstream; minor expressions have been described also in human brain [2], heart, kidney, testis and ovary. Afamin has been reported to bind vitamin E, especially α-tocopherol and γ-tocopherol, two of the most important forms of vitamin E, in vitro and in vivo and to possess multiple binding sites for both tocopherol isomers. Comparative proteomics has previously identified afamin as a potential biomarker for ovarian cancer. Patients with ovarian cancer displayed significantly decreased plasma concentrations of afamin by comparison to healthy controls and, very recently, extended by showing significant associations between afamin plasma concentrations and clinical outcomes (response to therapy and survival rates) were reported. Human plasma afamin is reported to be strongly associated with criteria for metabolic syndrome. Data from a prospective study design as well as corresponding data from afamin-transgenic mice suggest an active role of afamin in the development of metabolic syndrome [7]. In patients with polycystic ovary syndrome, afamin might serve as a discriminatory predictive parameter of insulin resistance and metabolic syndrome.

References to Product

References

  • Dieplinger H, Dieplinger B. Afamin - A pleiotropic glycoprotein involved in various disease states. Clin Chim Acta. 2015 Apr 16;446:105-110
References to Summary

References to Afamin

  • Jackson D, Craven RA, Hutson RC, Graze I, Lueth P, Tonge RP, Hartley JL, Nickson JA, Rayner SJ, Johnston C, Dieplinger B, Hubalek M, Wilkinson N, Perren TJ, Kehoe S, Hall GD, Daxenbichler G, Dieplinger H, Selby PJ, Banks RE. Proteomic profiling identifies afamin as a potential biomarker for ovarian cancer. Clin Cancer Res. 2007 Dec 15;13 (24):7370-9
  • Jerkovic L, Voegele AF, Chwatal S, Kronenberg F, Radcliffe CM, Wormald MR, Lobentanz EM, Ezeh B, Eller P, Dejori N, Dieplinger B, Lottspeich F, Sattler W, Uhr M, Mechtler K, Dwek RA, Rudd PM, Baier G, Dieplinger H. Afamin is a novel human vitamin E-binding glycoprotein characterization and in vitro expression. J Proteome Res. 2005 May-Jun;4 (3):889-99
  • Kim BJ, Lee YS, Lee SY, Park SY, Dieplinger H, Ryu SH, Yea K, Choi S, Lee SH, Koh JM, Kim GS. Afamin secreted from nonresorbing osteoclasts acts as a chemokine for preosteoblasts via the Akt-signaling pathway. Bone. 2012 Sep;51 (3):431-40
  • Kronenberg F, Kollerits B, Kiechl S, Lamina C, Kedenko L, Meisinger C, Willeit J, Huth C, Wietzorrek G, Altmann ME, Thorand B, Melmer A, Dahnhardt D, Santer P, Rathmann W, Paulweber B, Koenig W, Peters A, Adham IM, Dieplinger H. Plasma concentrations of afamin are associated with the prevalence and development of metabolic syndrome. Circ Cardiovasc Genet. 2014 Dec;7 (6):822-9
  • Melmer A, Fineder L, Lamina C, Kollerits B, Dieplinger B, Braicu I, Sehouli J, Cadron I, Vergote I, Mahner S, Zeimet AG, Castillo-Tong DC, Ebenbichler CF, Zeillinger R, Dieplinger H. Plasma concentrations of the vitamin E-binding protein afamin are associated with overall and progression-free survival and platinum sensitivity in serous ovarian cancer--a study by the OVCAD consortium. Gynecol Oncol. 2013 Jan;128 (1):38-43
  • Seeber B, Morandell E, Lunger F, Wildt L, Dieplinger H. Afamin serum concentrations are associated with insulin resistance and metabolic syndrome in polycystic ovary syndrome. Reprod Biol Endocrinol. 2014;12:88
  • Seeber BE, Czech T, Buchner H, Barnhart KT, Seger C, Daxenbichler G, Wildt L, Dieplinger H. The vitamin E-binding protein afamin is altered significantly in the peritoneal fluid of women with endometriosis. Fertil Steril. 2010 Dec;94 (7):2923-6
  • Seeber BE, Morandell E, Kurzthaler D, Wildt L, Dieplinger H. Afamin, a vitamin E-binding protein elevated in metabolic syndrome, is increased in women with polycystic ovary syndrome (PCOS) and insulin resistance. Hum Reprod . 2010;25 (suppl. 1):I302
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