Sandwich ELISA, HRP-labelled antibody
The ALPHA CrossLaps® (CTX-I) ELISA is an enzyme-linked immunosorbent assay for the quantification of non-isomerized fragments of C-terminal telopeptides of Type-I collagen (α CTX-I) in human urine. The ALPHA CrossLaps® (CTX-I) ELISA is intended for use as an indicator of degradation of non-isomerised bone collagen and may be used as an aid in the identification of skeletal metastases in patients with breast and prostate cancer. In addition, the ALPHA CrossLaps®(CTX-I) ELISA may be applied to humans as an aid in the diagnosis of Paget’s disease and the monitoring of anti-resorptive therapy in patients with Paget’s disease.
Type I collagen accounts for more than 90% of the organic matrix of bone and is synthesised primarily in bone. During renewal of the skeleton, type I collagen is degraded, and small peptide fragments are excreted into the urine. These fragments can be measured by the ALPHA CrossLaps® (CTX-I) ELISA. The ALPHA CrossLaps® (CTX-I) ELISA is based on one highly specific monoclonal antibody against the amino acid sequence of EKAHDGGR. In order to obtain a specific signal in the ALPHA CrossLaps® (CTX-I) ELISA, two chains of EKAHDGGR must be cross linked.
45 µL (excluding dead volume) 25 μL pre-diluted (1+7)
Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
0.80 ng/mL ALPHA CrossLaps