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Androstenedione ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Human
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Cat. No. Size Price


RCD001R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample Requirements

25 μl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–10 ng/ml

Limit of Detection

0.05 ng/ml

Intra-assay (Within-Run)

CV = 5.2%

Inter-assay (Run-to-Run)

CV = 8.7%

Spiking Recovery

101,00%

Dilutation Linearity

102,00%

Summary

Research topic

Steroid hormones

Summary

Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

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