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Manufactured by BioVendor

Androstenedione ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:A4- androstenedione, delta4 androstenedione
  • Species:Human
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Cat. No. Size Price

RCD001R 96 wells (1 kit)
PubMed Product Details
Technical Data


Competitive ELISA, Immobilized antigen



Sample Requirements

25 μl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–10 ng/ml

Limit of Detection

0.05 ng/ml

Intra-assay (Within-Run)

CV = 5.2%

Inter-assay (Run-to-Run)

CV = 8.7%

Spiking Recovery


Dilution Linearity



Research topic

Autoimmunity, Reproduction, Steroid hormones


Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

Product References (1)


  • Rodríguez-Gutiérrez R, Villarreal-Pérez JZ, Morales-Martinez FA,Rodríguez-Guajardo R, González-Saldivar G, Mancillas-Adame LG, Alvarez-VillalobosNA, Lavalle-Gonzalez FJ, González-González JG. Ovarian and adrenal androgens and their link to high human chorionic gonadotropin levels: a prospective controlled study. Int J Endocrinol. 2014;2014:191247. doi: 10.1155/2014/191247. Epub 2014Nov 23. PubMed PMID: 25505909; PubMed Central PMCID: PMC4258315. See more on PubMed
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