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Manufactured by BioVendor

Angiopoietin-Like Protein 3 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Angiopoietin-related protein 3, Angiopoietin-5, ANG-5, ANGPT5, UNQ153/PRO179, ANGPTL-3
  • Species:Human
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Cat. No. Size Price

RD191092200R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

30 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under this condition, the kit is stable until the expiration date (see the label on the box).

Calibration Curve

Calibration Range

12.5–400 ng/ml

Limit of Detection

1.08 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 3.7%

Inter-assay (Run-to-Run)

n = 6; CV = 8.9%

Spiking Recovery


Dilution Linearity



  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
  • monkey Yes (recommended dilution 1:3)


  • It is intended for research use only
  • The total assay time is less than 3.5 hours.
  • The kit measures Angptl3 in serum and plasma (EDTA, citrate, heparin).
  • Assay format - 96 wells
  • Quality controls are human serum based. No animal sera are used.
  • Standard is recombinant protein based.
  • Components of kit are provided ready to use, concentrated or lyophilized.

Research topic

Energy metabolism and body weight regulation, Oncology


Angiopoietin-like Protein 3 (Angptl3) is one from six members of Angiopoietin-like (Angptl) family of proteins, which has been identified as orphan ligands with structural similarity to angiopoietins. Angptl3 has 490-amino acid residues and its expression is restricted to the liver.
Angptl3 and Angptl4 have been shown to regulate fat, lipid or glucose metabolic homeostasis. Angptl3 is a hepatocyte-derived circulating factor that affect lipid metabolism and is involved in regulating lipid storage and breakdown.
Overexpression of Angptl3 or intravenous injection of the purified protein in mice elicited an increase in circulating plasma lipid levels. Angptl3 decreases very low density lipoprotein (VLDL) triglyceride clearance by inhibiting lipoprotein lipase (LPL) activity, directly targeting adipose cells to activate lipolysis. This results in an increased release of free fatty acid (FFA) and glycerol from adipocytes. These observations indicate that Angptl3 might regulate lipid metabolism by inhibiting LPL and by stimulating lipolysis in adipocytes.
Recent findings suggest that elevated levels of Angptl3 in diabetic states might be involved in inducing hypertriglyceridemia and hyperfattyacidemia in diabetes and obesity. Hypertriglyceridemia causes triglyceride accumulation in peripheral tissues such as skeletal muscles to enhance insulin- and leptin- resistance, and in vessel walls to induce atherosclerosis. Hyperfattyacidemia also affects the regulation of insulin secretion.
Taken together, these findings suggest that abnormalities in the regulation of Angptl3 might be involved in the pathogenesis of metabolic syndrome.
Angptl3 also suggest a possible role in the regulation of proliferation of new vessel from preexisting capillaries; a process termed angiogenesis also plays a key role in the progression of solid tumor growth, diabetic retinopathies, psoriasis, inflammation, and rheumathoid arthritis.

Areas of investigation: Lipid metabolism, Angiogenesis, Atherosclerosis

Product References (7)


  • Bužga M, Evžen M, Pavel K, Tomáš K, Vladislava Z, Pavel Z, Svagera Z. Effects of the intragastric balloon MedSil on weight loss, fat tissue, lipid metabolism, and hormones involved in energy balance. Obes Surg. 2014 Jun;24(6):909-15. doi:10.1007/s11695-014-1191-4. PubMed PMID: 24488758; PubMed Central PMCID:PMC4022986. See more on PubMed
  • Cinkajzlová A, Mráz M, Lacinová Z, Kloučková J, Kaválková P, Kratochvílová H, Trachta P, Křížová J, Haluzíková D, Škrha J, Papežová H, Haluzík M.Angiopoietin-like protein 3 and 4 in obesity, type 2 diabetes mellitus, andmalnutrition: the effect of weight reduction and realimentation. Nutr Diabetes.2018 Apr 25;8(1):21. doi: 10.1038/s41387-018-0032-2. PubMed PMID: 29695708;PubMed Central PMCID: PMC5916880. See more on PubMed
  • Muniyappa R, Abel BS, Asthana A, Walter MF, Cochran EK, Remaley AT, SkarulisMC, Gorden P, Brown RJ. Metreleptin therapy lowers plasma angiopoietin-likeprotein 3 in patients with generalized lipodystrophy. J Clin Lipidol. 2017 Mar - Apr;11(2):543-550. doi: 10.1016/j.jacl.2017.02.002. Epub 2017 Feb 24. PubMedPMID: 28502512; PubMed Central PMCID: PMC5433252. See more on PubMed
  • Robciuc MR, Tahvanainen E, Jauhiainen M, Ehnholm C. Quantitation of serumangiopoietin-like proteins 3 and 4 in a Finnish population sample. J Lipid Res.2010 Apr;51(4):824-31. doi: 10.1194/jlr.M002618. Epub 2009 Oct 13. PubMed PMID:19826106; PubMed Central PMCID: PMC2842146. See more on PubMed
  • Stejskal D, Karpísek M, Humenanská V, Solichová P, Stejskal P.Angiopoietin-like protein 3: development, analytical characterization, andclinical testing of a new ELISA. Gen Physiol Biophys. 2007 Sep;26(3):230-3.PubMed PMID: 18063851. See more on PubMed
  • Stitziel NO, Khera AV, Wang X, Bierhals AJ, Vourakis AC, Sperry AE, Natarajan P, Klarin D, Emdin CA, Zekavat SM, Nomura A, Erdmann J, Schunkert H, Samani NJ,Kraus WE, Shah SH, Yu B, Boerwinkle E, Rader DJ, Gupta N, Frossard PM, Rasheed A,Danesh J, Lander ES, Gabriel S, Saleheen D, Musunuru K, Kathiresan S; PROMIS and Myocardial Infarction Genetics Consortium Investigators. ANGPTL3 Deficiency andProtection Against Coronary Artery Disease. J Am Coll Cardiol. 2017 Apr25;69(16):2054-2063. doi: 10.1016/j.jacc.2017.02.030. Epub 2017 Apr 3. PubMedPMID: 28385496; PubMed Central PMCID: PMC5404817. See more on PubMed
  • Tarugi P, Bertolini S, Calandra S. Angiopoietin-like protein 3 (ANGPTL3)deficiency and familial combined hypolipidemia. J Biomed Res. 2019 Apr22;33(2):73-81. doi: 10.7555/JBR.32.20170114. PubMed PMID: 29752428; PubMedCentral PMCID: PMC6477171. See more on PubMed
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