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Annexin A1 Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35, ANXA1, ANX1, LPC1
  • Species:Human
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Cat. No. Size Price


RD172371100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 355 AA. MW: 39.8 kDa (calculated). UniProtKB acc.no. P04083 (Ala2-Asn346). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHASAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE Gel

14 % SDS-PAGE separation of Human Annexin A1:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in 20 mM Tris buffer, 50 mM NaCl, pH 8.

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Apoptosis, Atherosclerosis, Autoimmunity, Cardiovascular disease, Diabetology - Other Relevant Products, Immune Response, Infection and Inflammation, Neural tissue markers, Oncology, Renal disease, Neurodegenerative disease

Summary

Annexin A1 binds phospholipids and up to four calcium ions. It supports membrane fusion and takes part in exocytosis. It controles phospholipase A2 activity.

Summary References (14)

References to Annexin A1

  • Sheikh MH, Solito E. Annexin A1: Uncovering the Many Talents of an Old Protein. Int J Mol Sci. 2018 Mar 31;19(4). pii: E1045. doi: 10.3390/ijms19041045. Review. PubMed PMID: 29614751; PubMed Central PMCID: PMC5979524.
  • Purvis GSD, Solito E, Thiemermann C. Annexin-A1: Therapeutic Potential in Microvascular Disease. Front Immunol. 2019 Apr 30;10:938. doi: 10.3389/fimmu.2019.00938. eCollection 2019. Review. PubMed PMID: 31114582; PubMed Central PMCID: PMC6502989.
  • de Jong R, Leoni G, Drechsler M, Soehnlein O. The advantageous role of annexin A1 in cardiovascular disease. Cell Adh Migr. 2017 May 4;11(3):261-274. doi: 10.1080/19336918.2016.1259059. Epub 2016 Nov 18. Review. PubMed PMID: 27860536; PubMed Central PMCID: PMC5479459.
  • Qin C, Yang YH, May L, Gao X, Stewart AG, Tu Y, Woodman OL, Ritchie RH. Cardioprotective potential of annexin-A1 mimetics in myocardial infarction. Pharmacol Ther. 2015 Apr;148:47-65. doi: 10.1016/j.pharmthera.2014.11.012. Epub 2014 Nov 25. Review. PubMed PMID: 25460034.
  • Drechsler M, de Jong R, Rossaint J, Viola JR, Leoni G, Wang JM, Grommes J, Hinkel R, Kupatt C, Weber C, Döring Y, Zarbock A, Soehnlein O. Annexin A1 counteracts chemokine-induced arterial myeloid cell recruitment. Circ Res. 2015 Feb 27;116(5):827-35. doi: 10.1161/CIRCRESAHA.116.305825. Epub 2014 Dec 17. PubMed PMID: 25520364.
  • Qin CX, Rosli S, Deo M, Cao N, Walsh J, Tate M, Alexander AE, Donner D, Horlock D, Li R, Kiriazis H, Lee MKS, Bourke JE, Yang Y, Murphy AJ, Du XJ, Gao XM, Ritchie RH. Cardioprotective Actions of the Annexin-A1 N-Terminal Peptide, Ac(2-26), Against Myocardial Infarction. Front Pharmacol. 2019 Apr 3;10:269. doi: 10.3389/fphar.2019.00269. eCollection 2019. PubMed PMID: 31001111; PubMed Central PMCID: PMC6457169.
  • Ansari J, Kaur G, Gavins FNE. Therapeutic Potential of Annexin A1 in Ischemia Reperfusion Injury. Int J Mol Sci. 2018 Apr 16;19(4). pii: E1211. doi: 10.3390/ijms19041211. Review. PubMed PMID: 29659553; PubMed Central PMCID: PMC5979321.
  • Iyer S, Rikhi A, Adel F, Wan S-H, Chakraborty H, Tang W-H, Felker GM, Givertz M, Chen H. Annexin A1 a novel biomarker for congestion in acute heart failure? JACC. 2019; 73(9)Suppl 1. Doi: 10.1016/S0735-1097(19)31462-7.
  • Labes R, Dittert P, Bachmann S, Paliege A. Sources for anti-inflammatory annexin A1 signals during acute anti-Thy-1.1 nephritis. FASEB J. Epub 2016 Apr 1.
  • Ka SM, Tsai PY, Chao TK, Yang SM, Hung YJ, Chen JS, Shui HA, Chen A. Urine annexin A1 as an index for glomerular injury in patients. Dis Markers. 2014;2014:854163. doi: 10.1155/2014/854163. Epub 2014 Jan 20. PubMed PMID: 24591769; PubMed Central PMCID: PMC3925619.
  • Purvis GSD, Collino M, Loiola RA, Baragetti A, Chiazza F, Brovelli M, Sheikh MH, Collotta D, Cento A, Mastrocola R, Aragno M, Cutrin JC, Reutelingsperger C, Grigore L, Catapano AL, Yaqoob MM, Norata GD, Solito E, Thiemermann C. Identification of AnnexinA1 as an Endogenous Regulator of RhoA, and Its Role in the Pathophysiology and Experimental Therapy of Type-2 Diabetes. Front Immunol. 2019 Mar 27;10:571. doi: 10.3389/fimmu.2019.00571. eCollection 2019. PubMed PMID: 30972066; PubMed Central PMCID: PMC6446914.
  • Purvis GSD, Chiazza F, Collino M, Solito E, Thiemermann C. Endogenous Annexin-A1 is a Protective Determinant in HFD-induced Insulin Resistance and Diabetic Nephropathy. FASEB J. Epub 2017 Apr 1.
  • Purvis GSD, Chiazza F, Chen J, Azevedo-Loiola R, Martin L, Kusters DHM, Reutelingsperger C, Fountoulakis N, Gnudi L, Yaqoob MM, Collino M, Thiemermann C, Solito E. Annexin A1 attenuates microvascular complications through restoration of Akt signalling in a murine model of type 1 diabetes. Diabetologia. 2018 Feb;61(2):482-495. doi: 10.1007/s00125-017-4469-y. Epub 2017 Oct 30. PubMed PMID: 29085990; PubMed Central PMCID: PMC6448955.
  • McArthur S, Cristante E, Paterno M, Christian H, Roncaroli F, Gillies GE, Solito E. Annexin A1: a central player in the anti-inflammatory and neuroprotective role of microglia. J Immunol. 2010
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