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Annexin A2 Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:Annexin II, Annexin-2, Calpactin I heavy chain, Calpactin-1 heavy chain, Chromobindin-8, Lipocortin II, Placental anticoagulant protein IV, PAP-IV, Protein I, p36, ANX2, ANX2L4, CAL1H, LPC2D
  • Species:Human
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Cat. No. Size Price


RD172348100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 347 AA. MW: 39.6 kDa (calculated). UniProtKB acc.no. P07355 (Ser2-Asp339). N-terminal His-tag (9 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHASTVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Source

E. coli

Purity

Purity as determined by densitometric image analysis: >95%

SDS-PAGE Gel

14 % SDS-PAGE separation of Human Annexin A2:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0.4 μm) and lyophilized in phosphate buffered saline pH=7.0, 1% (w/v) Sucrose, 4% (w/v) Mannitol

Reconstitution

Add 200 µl of deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Oncology, Others

Summary

Annexin A2 is a 36-kDa protein produced by endothelial cells, monocytes, macrophages, trophoblast cells, and some tumor cells and exists both free in the cytoplasm and in association with intracellular and plasma membrane surfaces. Annexin A2 belongs to the annexin family of Ca2+-regulated phospholipid binding proteins, which are expressed in plants, animals, and protists throughout the phylogenetic tree. Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. Annexin A2 forms heterotetrameric complex containing the S100A10 dimer and two annexin A2 chains significantly alters the properties of this annexin in vitro and also within cells. Importantly, the annexin A2-S100A10 complex can aggregate membrane vesicles at micromolar Ca2+ levels, a property not shared with monomeric annexin A2 or, as a matter of fact, any other annexin. Annexin A2 plays a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.

Summary References (7)

References to Annexin A2

  • Deng S, Jing B, Xing T, Hou L, Yang Z. Overexpression of annexin A2 is associated with abnormal ubiquitination in breast cancer. Genomics Proteomics Bioinforma. 2012 Jun;10 (3):153-7
  • Flood EC, Hajjar KA. The annexin A2 system and vascular homeostasis. Vascul Pharmacol. 2011 Mar-Jun;54 (3-6):59-67
  • Gerke V, Moss SE. Annexins: from structure to function. Physiol Rev. 2002 Apr;82 (2):331-71
  • Martin M, Leffler J, Blom AM. Annexin A2 and A5 serve as new ligands for C1q on apoptotic cells. J Biol Chem. 2012 Sep 28;287 (40):33733-44
  • Rodrigo Tapia JP, Pena Alonso E, Garcia-Pedrero JM, Florentino Fresno M, Suarez Nieto C, Owen Morgan R, Fernandez MP. [Annexin A2 expression in head and neck squamous cell carcinoma]. Acta Otorrinolaringol Esp. 2007 Jun-Jul;58 (6):257-62
  • Sarkar S, Maxwell CA, Luthra G, Singal A, Qiu S, Bauer VP, Okorodudu AO, Singh P. Annexina2 is Increasingly Expressed and Released Into the Serum of Patients Positive for Colonic Growths/Tumors in Relation to Disease Progression: Diagnostic Implications. Gastroenterology. 2011 May;140 (5):341
  • Xin H, Zhang Y, Wang H, Sun S. Alterations of profibrinolytic receptor annexin A2 in pre-eclampsia: a possible role in placental thrombin formation. Thromb Res. 2012 May;129 (5):563-7
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