Annexin A2 NATIVE, Human Adipose Tissue

Cat. No.	Size	Price
RD165348025	0.025 mg

Technical Data

Type

Native protein

Description

Native protein isolated from Human Adipose Tissue, 338 AA, MW 38,472 kDa (calculated without glycosylation). Protein identity confirmed by MALDI-TOF/TOF (NCBI no. gi|4757756).

Amino Acid Sequence

STVHEILCKLSLEGDHSTPPSAYGSVKAYTNFDAERDALNIETAIKTKGVDEVTIVNILTNRSNAQRQDIAFAYQRRTKKELASALKSALSGHLETVILGLLKTPAQYDASELKASMKGLGTDEDSLIEIICSRTNQELQEINRVYKEMYKTDLEKDIISDTSGDFRKLMVALAKGRRAEDGSVIDYELIDQDARDLYDAGVKRKGTDVPKWISIMTERSVPHLQKVFDRYKSYSPYDMLESIRKEVKGDLENAFLNLVQCIQNKPLYFADRLYDSMKGKGTRDKVLIRIMVSRSEVDMLKIRSEFKRKYGKSLYYYIQQDTKGDYQKALLYLCGGDD

Source

Human adipose tissue

Purity

Purity as determined by densitometric image analysis: >90 %

SDS-PAGE Gel

SDS-PAGE analysis of Annexin A2 NATIVE, Human Adipose Tissue 12% gel stained with Coomassie Brillant Blue R250

M.W. marker – 14, 21, 31, 45, 66, 97 kDa
reduced and boiled sample, 2.5μg/lane
non reduced and non boiled sample, 2.5μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0,5 mg/mL in 0,05M phosphate buffer, 0,075M NaCl, pH 6,5.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies, Immunological methods

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Oncology, Others

Summary

Annexin A2 is a 36-kDa protein produced by endothelial cells, monocytes, macrophages, trophoblast cells, and some tumor cells and exists both free in the cytoplasm and in association with intracellular and plasma membrane surfaces. Annexin A2 belongs to the annexin family of Ca2+-regulated phospholipid binding proteins, which are expressed in plants, animals, and protists throughout the phylogenetic tree. Annexins possess a series of unique 70 amino acid annexin-repeat modules that form a curved disk and allow for the creation of an intercessory complex between plasma membrane and cytofilaments. Annexin A2 forms heterotetrameric complex containing the S100A10 dimer and two annexin A2 chains significantly alters the properties of this annexin in vitro and also within cells. Importantly, the annexin A2-S100A10 complex can aggregate membrane vesicles at micromolar Ca2+ levels, a property not shared with monomeric annexin A2 or, as a matter of fact, any other annexin. Annexin A2 plays a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.

Summary References (7)

References to Annexin A2

Deng S, Jing B, Xing T, Hou L, Yang Z. Overexpression of annexin A2 is associated with abnormal ubiquitination in breast cancer. Genomics Proteomics Bioinforma. 2012 Jun;10 (3):153-7
Flood EC, Hajjar KA. The annexin A2 system and vascular homeostasis. Vascul Pharmacol. 2011 Mar-Jun;54 (3-6):59-67
Gerke V, Moss SE. Annexins: from structure to function. Physiol Rev. 2002 Apr;82 (2):331-71
Martin M, Leffler J, Blom AM. Annexin A2 and A5 serve as new ligands for C1q on apoptotic cells. J Biol Chem. 2012 Sep 28;287 (40):33733-44
Rodrigo Tapia JP, Pena Alonso E, Garcia-Pedrero JM, Florentino Fresno M, Suarez Nieto C, Owen Morgan R, Fernandez MP. [Annexin A2 expression in head and neck squamous cell carcinoma]. Acta Otorrinolaringol Esp. 2007 Jun-Jul;58 (6):257-62
Sarkar S, Maxwell CA, Luthra G, Singal A, Qiu S, Bauer VP, Okorodudu AO, Singh P. Annexina2 is Increasingly Expressed and Released Into the Serum of Patients Positive for Colonic Growths/Tumors in Relation to Disease Progression: Diagnostic Implications. Gastroenterology. 2011 May;140 (5):341
Xin H, Zhang Y, Wang H, Sun S. Alterations of profibrinolytic receptor annexin A2 in pre-eclampsia: a possible role in placental thrombin formation. Thromb Res. 2012 May;129 (5):563-7

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