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Apo B-48 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Apolipoprotein B-48
  • Species:Human
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RSHAKHB48R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin

Sample Requirements

50 µl/well (Diluted to 100X with buffer solution ©)

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2.5–160 ng/ml

Limit of Detection

0.198 ng/ml

Intra-assay (Within-Run)

n = 5; CV = 3.5%

Inter-assay (Run-to-Run)

n = 3; CV = 5.7%

Spiking Recovery

99.9 %

Summary

Features

  • This is intended for research use only.
  • Rapid assay (total reaction time: 2 h 50min.).
  • This kit is for apolipoprotein B-48 in human serum or plasma (citric acid for plasma is not suitable).
  • A small sample volume of serum or plasma (5 µl) is needed.
  • Assay format is 96 wells.
  • Standard Apo B-48 is derived from human.

Research topic

Cardiovascular disease, Energy metabolism and body weight regulation, Lipoprotein metabolism

Summary

Plasma lipoproteins are complex substances composed of certain ratios of lipids and proteins, and are important factors for serum lipids transportation. On the surface of the lipoprotein structure, apolipoproteins are present and playing roles in stabilization of lipoprotein structure, in activation of enzymes relating to lipoprotein metabolism, and in binding with lipoprotein receptors on the cell surface. Apolipoprotein-B48 (ApoB-48) has 48% amino acid sequence of apolipoprotein B-100 which is present in lipoproteins ,VLDL, LDL and HDL of liver origin. ApoB-48 is an apolipoprotein specific to a lipoprotein, chylomicron (CM) which is formed in intestine and carries exogenous lipids derived from foods to the liver and peripheral tissues. Measurement of ApoB-48 is useful in observation and pursuit of postprandial dynamics of lipoprotein, lipid-soluble nutrients and drugs.

References to Product

References

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  • Beigneux AP, Franssen R, Bensadoun A, Gin P, Melford K, Peter J, Walzem RL, Weinstein MM, Davies BS, Kuivenhoven JA, Kastelein JJ, Fong LG, Dallinga-Thie GM, Young SG. Chylomicronemia with a mutant GPIHBP1 (Q115P) that cannot bind lipoprotein lipase. Arterioscler Thromb Vasc Biol. 2009 Jun;29 (6):956-62
  • Castro Cabezas M, Erkelens DW, Kock LA, De Bruin TW. Postprandial apolipoprotein B100 and B48 metabolism in familial combined hyperlipidaemia before and after reduction of fasting plasma triglycerides. Eur J Clin Invest. 1994 Oct;24 (10):669-78
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  • Kinoshita M, Kojima M, Matsushima T, Teramoto T. Determination of apolipoprotein B-48 in serum by a sandwich ELISA. Clin Chim Acta. 2005 Jan;351 (1-2):115-20
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  • Nakajima K, Nakajima Y, Takeichi S, Fujita MQ. ApoB-100 carrying lipoprotein, but not apoB-48, is the major subset of proatherogenic remnant-like lipoprotein particles detected in plasma of sudden cardiac death cases. Atherosclerosis. 2007 Oct;194 (2):473-82
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