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Apolipoprotein M Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Apo M, Protein G3a, G3a, G3A, NG20
  • Species:Human
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Cat. No. Size Price


RD191129200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma (EDTA, citrate, heparin)

Sample Requirements

20 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.313–10 ng/ml

Limit of Detection

0.067 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 5.1%

Inter-assay (Run-to-Run)

n = 5; CV = 5.7%

Spiking Recovery

107,60%

Dilutation Linearity

92,40%

Summary

Features

  • For research use only!
  • The total assay time is less than 3 hours.
  • The kit measures total Apolipoprotein M in serum and plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Standard is recombinant protein.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Immune Response, Infection and Inflammation, Lipoprotein metabolism

Summary

Human Apolipoprotein M (ApoM, protein G3a, NG20-like protein NG20), is a protein that in humans is encoded by the APOM gene. Two different isoforms have been found for this gene. ApoM is a secreted 25kDa member of the lipocalin protein family consisting of 188 amino acids. Human APOM shares sequence identity with pig (91%) and mouse (80%) APOM. The apolipoproteins are a structurally-unrelated group of proteins that all have some role in the transport of lipids in blood. Apolipoproteins plus phospholipids, cholesterol and triglycerides, form spherical particles with a lipid/hydrophobic center and a (apolipo)protein coat. The apolipoprotein coat promotes aqueous solubility and serves as a ligand for lipoprotein receptors. HDL may contain apoliporoteins A,C, D,E, J, L and M, while LDL contains apolipoproteins B and E.

Areas of investigation: Lipoprotein metabolism, Obesity, Atherosclerosis, Diabetes mellitus, Immune Response, Infection and Inflammation.

References to Product

References

  • Engin-Ustun Y, Caglayan EK, Gocmen AY, Polat MF. Postmenopausal Osteoporosis Is Associated with Serum Chemerin and Irisin but Not with Apolipoprotein M Levels. J Menopausal Med. 2016 Aug;22 (2):76-9
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