Type
Sandwich ELISA, HRP-labelled antibody
Applications
Serum, Cerebrospinal fluid
Sample Requirements
25 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Place the lyophilized Master Standards and Quality Controls at –20 °C after the kit delivery. Store the other kit components at 2–8°C. Under these conditions the kit is stable till the expiry date is over. (See the expiry date indicated on the kit label).
Calibration Curve
Calibration Range
5–320 ng/ml
Limit of Detection
2.7 ng/ml
Intra-assay (Within-Run)
n = 8; CV = 5.8%
Inter-assay (Run-to-Run)
n = 8; CV = 7.8%
Spiking Recovery
87,00%
Dilution Linearity
91,00%
Crossreactivity
- bovine Non-detectable
- cat Yes (recommended dilution 1:4)
- dog Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- sheep Non-detectable
- chicken Not tested
- human Yes
- monkey Yes (recommended dilution 1:4)
Features
- It is intended for research use only
- The total assay time is less than 3 hours
- The kit measures total Arginase I (Liver-Type) in serum and cerebrospinal fluid (CSF)
- Assay format is 96 wells
- Standard is recombinant protein based
- Serum samples require very careful preparation. The erythrocytes have to be spinned down immediately (within few seconds) after taking blood to avoid hemolysis and contamination of the sample with erythrocyte arginase
- Components of the kit are provided ready to use, concentrated or lyophilized
Research topic
Asthma and allergic rhinitis, Blood pressure regulation and NO metabolism, Immunology, Oncology, Pulmonary diseases
Summary
Arginase [EC 3.5.3.1; L-arginine aminohydrolase] is an enzyme that hydrolyzes Larginine to L-ornithine and urea in the urea cycle. Two forms of arginase exists which are designed as arginase I and arginase II. Liver-type arginase I is expressed primarily in the liver and to some extend in the erythrocytes. Arginase II is expressed in many extrahepatic tissues, such as brain, spinal cord, kidney, small intestine and mammary gland. Although arginase I and arginase II have similar enzyme activities, they have different pI, immunological reactivity and are encoded by different genes. Human arginase I is a 35 kDa protein circulating in blood probably as a homotrimer. Circulating liver-type arginase was clinically used as a liver specific marker which may reflect not only early occurrence of liver injury but also early termination of liver injury. The measurement of liver-type arginase is clinically applicable for monitoring conditions of patients with liver disorders or pre- and postoperative conditions of patients who received partial hepatectomy with quicker normalization in comparison with aminotransferases (ALT and AST). Recently, arginase I gene was found to be one of the most prominent among astma genes. In situ hybridization demonstrated marked staining of arginase I in submucosal inflammatory lesions and arginase activity increased in allergen challenged lungs. Finally, it was found that both arginase I was the most significantly up-regulated protein in the murine spinal cord during experimental autoimmune encephalomyelitis. The results indicated that arginase I played important roles in autoimmune inflammation in the central nervous system.
