Total 159 AA. MW: 18.0 kDa (calculated). UniProtKB acc. no. Q9Y275 soluble form (Ala134–Leu285). N-terminal His-tag (7 extra AA). Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
Purity as determined by densitometric image analysis: > 95%
12 % SDS-PAGE separation of Human BAFF (E.coli):
- M.W. marker – 14, 21, 31, 45, 66, 97 kDa
- Reduced and boiled sample, 2.5 μg/lane
- Non-reduced and non-boiled sample, 2.5 μg/lane
< 0.1 EU/µg
Filtered (0.4 μm) and frozen from 0.3- 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 1% (w/v) sacharose, 4% (w/v) mannitol, pH 7.5.
Defrost at ambient temperature
Western blotting, ELISA
On ice. Upon receipt, store the product at the temperature recommended below.
Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles. The thawed protein can be stored at 4 °C for three days.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
This product is intended for research use only.