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Manufactured by BioVendor

BAFF Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant
  • Source:E. coli
  • Other names:B cell Activating Factor belonging to the TNF family, TNFSF13B, TNFSF20, TALL-1, BLyS, THANK, ZNTF4,Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator, BLyS, B-cell-activating factor, Dendritic cell-derived TNF-like molecule, TNF- and APOL-related leukocyte expressed ligand 1, TALL-1, CD_antigen: CD257
  • Species:Human
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New RD172584100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant

Description

Total 159 AA. MW: 18.0 kDa (calculated). UniProtKB acc. no. Q9Y275 soluble form (Ala134–Leu285). N-terminal His-tag (7 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MHHHHHHAVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL

Source

E. coli

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE Gel

14 % SDS-PAGE separation of Human BAFF (E.coli):

  1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
  2. Reduced and boiled sample, 2.5 μg/lane
  3. Non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/µg

Formulation

Filtered (0.4 μm) and frozen from 0.3- 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 1% (w/v) sacharose, 4% (w/v) mannitol, pH 7.5.

Reconstitution

Defrost at ambient temperature

Applications

Western blotting, ELISA

Shipping

On ice. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles. The thawed protein can be stored at 4 °C for three days.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

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