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Betatrophin Mouse E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Other names:RIFL, Hepatocellular carcinoma-associated protein TD26, C19orf80, UNQ599/PRO1185, Lipasin, Angiopoietin-like protein 8, ANGPTL-8, ANGPTL 8, ANGPTL8
  • Species:Mouse
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Cat. No. Size Price


RD272347100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 193 AA. MW: 21.8 kDa (calculated). UniProtKB acc.no. Q8R1L8 (Val16-Ala198). N-terminal His-tag (10 extra AA). Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

MKHHHHHHASVRPAPVAPLGGPEPAQYEELTLLFHGALQLGQALNGVYRATEARLTEAGHSLGLYDRALEFLGTEVRQGQDATQELRTSLSEIQVEEDALHLRAEATARSLGEVARAQQALRDTVRRLQVQLRGAWLGQAHQEFETLKARADKQSHLLWALTGHVQRQQREMAEQQQWLRQIQQRLHTAALPA

Source

E. coli

Purity

Purity as determined by densitometric image analysis: >95%

SDS-PAGE Gel

14 % SDS-PAGE separation of Mouse Betatrophin:
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and boiled sample, 2.5 μg/lane
3. non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/mL solution in 30 mM acetate buffer, pH 4.

Reconstitution

Add 0.1 M acetate buffer pH 4.0 to prepare a working stock solution of 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 μg/mL. In higher concentrations the solubility of this antigen is limited. Filter sterilize your culture media/working solutions containing this non-sterile product before using in cell culture.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Lipoprotein metabolism, Animal studies

Summary

Lipasin (RIFL) is an important new regulator of lipid metabolism that regulates serum triglyceride levels. It is a member of ANGPTL protein family.

Summary References (9)

References to Betatrophin

  • Espes D, Lau J, Carlsson PO. Increased circulating levels of betatrophin in individuals with long-standing type 1 diabetes. Diabetologia. 2014 Jan;57 (1):50-3
  • Espes D, ORCID: 0000-0001-8843-7941, Martinell M, ORCID: 0000-0002-6060-6229, Carlsson PO, ORCID: 0000-0002-7666-073X. Increased circulating betatrophin concentrations in patients with type 2 diabetes. Int J Endocrinol. 2014;2014:323407
  • Jiao Y, Le Lay J, Yu M, Naji A, Kaestner KH. Elevated mouse hepatic betatrophin expression does not increase human beta-cell replication in the transplant setting. Diabetes. 2014 Apr;63 (4):1283-8
  • Raghow R. Betatrophin: A liver-derived hormone for the pancreatic beta-cell proliferation. World J Diabetes. 2013 Dec 15;4 (6):234-7
  • Salas E, Rabhi N, Froguel P, Annicotte JS. Role of Ink4a/Arf locus in beta cell mass expansion under physiological and pathological conditions. J Diabetes Res. 2014;2014:873679
  • Trebotic LK, Klimek P, Thomas A, Fenzl A, Leitner K, Springer S, Kiefer FW, Kautzky-Willer A. Circulating Betatrophin Is Strongly Increased in Pregnancy and Gestational Diabetes Mellitus. PLoS One. 2015;10 (9):e0136701
  • Wang Y, Quagliarini F, Gusarova V, Gromada J, Valenzuela DM, Cohen JC, Hobbs HH. Mice lacking ANGPTL8 (Betatrophin) manifest disrupted triglyceride metabolism without impaired glucose homeostasis. Proc Natl Acad Sci U S A. 2013 Oct 1;110 (40):16109-14
  • Yi P, Park JS, Melton DA. Betatrophin: a hormone that controls pancreatic beta cell proliferation. Cell. 2013 May 9;153 (4):747-58
  • Zhang R, Abou-Samra AB. A dual role of lipasin (betatrophin) in lipid metabolism and glucose homeostasis: consensus and controversy. Cardiovasc Diabetol. 2014;13:133
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