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BlitzAmp Hotstart qPCR Master Mix - Hi-ROX (800)

  • Type:BlitzAmp qPCR Master Mix
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New 1204214 800 Reactions
Product Details
Technical Data

Type

BlitzAmp qPCR Master Mix

Description

Qty of 4 x #1204212. 8 x 1,000 µl of BlitzAmp Hotstart qPCR Master Mix (2x), 800 x 20 µl reactions, For High-Rox qPCR instruments

MiRXES formulated BlitzAmp qPCR Master Mix to support research and routine testing requirements for high specificity, sensitivity, robustness and speed. Its proprietary blend of PCR polymerase, dNTPs, DNA binding dye, salts and stabilizers is the ideal molecular solution to generate high-quality data in the shortest time with ease.

Shipping

On frozen gel ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Unopened products are stable at -20°C until the expiry date printed on the label.

Summary

Summary

Turbocharge your Research with Lightning Fast PCR

  • Robust amplification of amplicon up to 250bp for difficult assays
  • Sensitive detection of low abundance targets
  • Highly reproducible data
  • Highly stable at room temperature
  • High tolerance for inhibitors and varying assay melting temperature
  • Highly versatile; designed to support most real-time qPCR machines in the market

MiRXES formulated BlitzAmp qPCR Master Mix to support research and routine testing requirements for high specificity, sensitivity, robustness and speed. Its proprietary blend of PCR polymerase, dNTPs, DNA binding dye, salts and stabilizers is the ideal molecular solution to generate high-quality data in the shortest time with ease.

Generate top Quality data in a Flash

  • Short qPCR running protocol - 34 minutes
  • Translate into higher productivity and notable capital investment savings

Fast and Robust

MiRXES BlitzAmp Master Mix supports robust amplification of amplicons up to 250 bp using Lightning cycling protocol for accurate quantification in just 34 min.

Amplicons of lengths 101 bp and 254 bp were amplified using qPCR Master Mixes.
Using the Lightning thermal cycling protocol (i.e., Hot start 95°C/5 min, denaturation 98°C/1 s, annealing/extension 60°C/5 s, for 40 cycles, total 34 min run time). In just 34 min run time, MiRXES BlitzAmp Master Mix supports efficient PCR amplification of up to 254 bp amplicon. Initial concentrations ranged from 5 x101 to 5 x107 copies per reaction.

Reaction Stability

Reactions set up with MiRXES BlitzAmp qPCR Master Mix can be stored at room temperature for up to 96 hours with neither decrease in performance nor increase in non-specific signals, making it ideal for high throughput laboratory setups.

Reaction mixes containing BlitzAmp qPCR Master Mix were left at room temperature after set up for up to 96 hours prior to qPCR to demonstrate superior reagent stability. Data showed that qPCR efficiency was maintained between 95-96% and Ct values were virtually identical for all conditions tested (standard deviations plotted above). No signals from NTC wells was detected.

Compatible with most Real-time qPCR machines

  • MiRXES BlitzAmp qPCR Master Mix is designed to support most real-time qPCR machines in the market
  • Lo-ROX and Hi-ROX

BlitzAmp Comparison Panel: Designed & Built for Scientific Comparison

  • Enabling user to assess the PCR efficiency and dynamic range of the qPCR Master Mix
  • Evaluate the qPCR Master Mix's robustness across small and large amplicon amplification
  • Supports different qPCR Platforms

BlitzAmp cDNA Synthesis Kit

  • Provide access to full spectrum of the transcriptome
  • Combining an optimized blend of random hexamers and anchored oligo dT primers
  • 8 logs of dynamic range
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