Block Ace is a novel blocking agent prepared from lactprotein. Since lactprotein contains a lot of hydrophobic groups, it shows a high affinity to plastic, membrane filter, etc. Block Ace exhibits an enhanced blocking effect on nonspecific adsorption of antigens or antibodies in the ELISA, RIA, and immunoblocking methods compared with the conventional blocking agents such as bovine serum albumin (BSA).
Superior to 1% BSA, Block Ace is a high-performance blocking reagent for ELISA assays and Western blotting. In comparison to BSA, Block Ace provides reduced backgrounds and sharper standard curves. It can also be used as a wash buffer and for diluting antibodies.
・Since this product has high blocking effects and maintains the background at a low level, it is useful for low-concentration measurement such as ELISA. ・Heat-treated: bovine-derived antibodies, enzymes, and viruses are inactivated. ・Stable for heat and freezing. ・Can stabilize physiologically active substances and can be used as a diluting agent. ・Compared with BSA solution, it is economical. ・It can be rapidly dissolved in water. ・Reliablie, consisten formulation.
Four (4) grams of Block Ace powder is dissolved into 100 mL of purified water, and this solution is used as a concentrate.
Blocking: For blocking purpose, either concentrate or 2-4x diluted solution with purified water is used (4x diluted solution is used for ELISA; concentrate is used for Western Blotting).
Dilution: For dilution of samples and labelled antibody, it is diluted to 10x with purified water.
Washing: For washing operation such as B/F separation, it is diluted to 10x with purified water, and the required volume of Tween 20 (0.05%–0.2%) is added.
Western blotting, ELISA, Immunoassays
On frozen gel ice packs. Upon receipt, store the product at the temperature recommended below.
Store at room temperature. (This product is heat treated, but there is no preservative or antibiotic. Once dissolved, it should be frozen before storage).
White lyophilized (freeze-dried) powder.
This product is furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.