Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The BMP-2 is purified by proprietary chromatographic techniques.
Amino Acid Sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Ala-Lys-His.
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
1. The ED50 as determined by the cytolysis of MC3T3-E1 cells is < 50 ng/ml. 2. When implanted in the mouse thighbone muscle and grow for 14 days, Muscle tissue was harvested and examined biochemically for calcium uptake. Our unit defined as 1 microgram calcium absorbed. The specific activity is more than 1000 Units/mg. 3. The activity of BMP-2 was tested using alkaline phosphatase, the activity in mouse muscle myoblast C2C12 cell line was found to be 1×105 U/mg protein. 4. The ectopic osteogenesis capacity is 1000mg fresh bone per 1mg of BMP-2.
BMP-2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 20mM NaAc pH=4.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution BMP2 should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Sterile filtered white lyophilized (freeze-dried) powder.