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Caspase-1 Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Interleukin-1ß-Converting Enzyme
  • Species:Mouse
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Cat. No. Size Price


RAG016R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.156–10 ng/ml

Limit of Detection

33 pg/ml

Summary

Research topic

Animal studies, Apoptosis, Immune Response, Infection and Inflammation

Summary

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin- 1β (IL-1β) and interleukin-18 (IL-18) and induces pyroptotic cell death. Caspase-1 is synthesized as inactive pro-Caspase-1, wich is activated and processed by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3/NALP3 responds to a variety of agonists including crystals. Pro-Caspase-1, once activated by an inflammasome complex is processed into p20 and p10 subunits that assembles to form a p20-p20 /p10-p10 active complex that is secreted by a nonconventional (non-ER-dependent) mechanism.

References to Summary

References to Caspase-1

  • Gross O. Measuring the inflammasome. Methods Mol Biol. 2012;844:199-222
  • Hornung V, Ablasser A, Charrel-Dennis M, Bauernfeind F, Horvath G, Caffrey DR, Latz E, Fitzgerald KA. AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC. Nature. 2009 Mar 26;458 (7237):514-8
  • Keller M, Ruegg A, Werner S, Beer HD. Active caspase-1 is a regulator of unconventional protein secretion. Cell. 2008 Mar 7;132 (5):818-31
  • Levinsohn JL, Newman ZL, Hellmich KA, Fattah R, Getz MA, Liu S, Sastalla I, Leppla SH, Moayeri M. Anthrax lethal factor cleavage of Nlrp1 is required for activation of the inflammasome. PLoS Pathog. 2012;8 (3):e1002638
  • Nickel W, Rabouille C. Mechanisms of regulated unconventional protein secretion. Nat Rev Mol Cell Biol. 2009 Feb;10 (2):148-55
  • Schroder K, Tschopp J. The inflammasomes. Cell. 2010 Mar 19;140 (6):821-32
  • Tschopp J, Schroder K. NLRP3 inflammasome activation: The convergence of multiple signalling pathways on ROS production?. Nat Rev Immunol. 2010 Mar;10 (3):210-5
  • Yazdi AS, Guarda G, D'Ombrain MC, Drexler SK. Inflammatory caspases in innate immunity and inflammation. J Innate Immun. 2010;2 (3):228-37
  • Zhao Y, Yang J, Shi J, Gong YN, Lu Q, Xu H, Liu L, Shao F. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 2011 Sep 29;477 (7366):596-600
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