Total 95 AA. MW: 11,2 kDa (calculated). Biotinylated at the last Lysine in sequence. UniProtKB acc.no# P80098 (24–99) Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
> 97% by SDS PAGE
EC50 = 1.4–2.6 nM determined by migration assay with cells expressing recombinant CCR2
<0.01 EU per 1µg of the protein
Add sterile water to prepare a working stock solution of approximately 0.1 mg/ml and let the lyophilized pellet dissolve completely.
Western blotting, Immunocytochemistry, ELISA, Cell culture and/or animal studies, Biologically active protein, Flow cytometry
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the lyophilized protein at –20 to –70 °C. Lyophilized protein remains stable until the expiry date when stored at-20 to –70 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -20 to –70 °C, the protein is stable for 3 months at –20 to –70 °C under sterile conditions after reconstitution. Reconstituted protein can be stored at 4 °C for 1 month under sterile conditions after reconstitution
Quality Control Test
Quantification is done by A280, using the calculated extinction coefficient (14,180 M-1 cm-1).
SDS-PAGE to determine purity of the protein.
LAL TEST to determine endotoxin level.
This product is intended for research use only.