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CD14 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cluster of differentiation 14
  • Species:Human
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Cat. No. Size Price


RGP001R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

100 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

6.8–220 ng/ml

Limit of Detection

< 6 ng/ml

Summary

Research topic

Cell surface proteins (sCD)

Summary

CD14 is a membrane-associated glycosylphospha­tidylinositol-linked protein expressed at the surface of cells, especially macrophages. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll- like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide. Soluble CD14 (sCD14), a 55-kDa glycoprotein found in plasma, has been shown to act as a shuttle for bacterial LPS and phospholipids, transporting LPS and phospholipid monomers from LPS aggregates or liposomes to high density lipoprotein particles. sCD14 has also been shown to mediate the transport of LPS and phosphatidyli­nositol into cells.

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