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Manufactured by BioVendor

CRP Canine HEK293 Tag free

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:HEK293
  • Other names:C-Reactive Protein
  • Species:Canine
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New RD472586100 0.1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Total 204 AA. MW: 23.2 kDa (calculated). NCBI Reference Sequence: NP_001301045.1. Protein identity confirmed by LC-MS/MS.

Amino Acid Sequence

QIDLDEKAFVFPRESENSYVILFPQLQKPMKAFTVCLQVYTDLTRPHSLFSYATKSQSNEILLFKERPGLFSVSVGGSDAFINFPQKFYAPQHFCVTWESVTGLTELWVDGKPMVRASLRRGYTVGSGASIVLGQEQDSFGGGFDKNQSLVGDIEDVNMWDFVLSPSQILTLYTTRALSPNVLNWRNLRYETRGEVFLKKELWS

Source

HEK293

Purity

Purity as determined by densitometric image analysis: > 95%

SDS-PAGE Gel

12 % SDS-PAGE separation of Canine CRP tagless (HEK):

  1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
  2. Reduced and boiled sample, 2.5 μg/lane
  3. Non-reduced and non-boiled sample, 2.5 μg/lane

Endotoxin

< 0.1 EU/μg

Formulation

Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 1mM CaCl2, pH 7.5.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.

Applications

Western blotting, ELISA

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store lyophilized protein at –80°C. Lyophilized protein remains stable until the expiry date when stored at –80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

This product is intended for research use only.

Summary

Research topic

Immune Response, Infection and Inflammation, Sepsis, Animal studies

Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

References to Summary

References to CRP

  • Gewurz H, Mold C, Siegel J, Fiedel B. C-reactive protein and the acute phase response. Adv Intern Med. 1982;27:345-72
  • Helgeson NGP, Adamson DM, Pike RB, James DS, Nicodemus DS, Lee BA, Miller GW. C-Reactive Protein : Laboratory Medicine, Vol. 2 (Race G. J., Ed.). Harper & Row, Hagerstown, chap. 1973;
  • Johnson HL, Chiou CC, Cho CT. Applications of acute phase reactants in infectious diseases. J Microbiol Immunol Infect. 1999 Jun;32 (2):73-82
  • Powell LJ. C-reactive protein--a review. Am J Med Technol. 1979 Feb;45 (2):138-42
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