Type
Recombinant protein
Description
Total 204 AA. MW: 23.2 kDa (calculated). NCBI Reference Sequence: NP_001301045.1. Protein identity confirmed by LC-MS/MS.
Amino Acid Sequence
QIDLDEKAFVFPRESENSYVILFPQLQKPMKAFTVCLQVYTDLTRPHSLFSYATKSQSNEILLFKERPGLFSVSVGGSDAFINFPQKFYAPQHFCVTWESVTGLTELWVDGKPMVRASLRRGYTVGSGASIVLGQEQDSFGGGFDKNQSLVGDIEDVNMWDFVLSPSQILTLYTTRALSPNVLNWRNLRYETRGEVFLKKELWS
Source
HEK293
Purity
Purity as determined by densitometric image analysis: > 95%
SDS-PAGE Gel
12 % SDS-PAGE separation of Canine CRP tagless (HEK):
- M.W. marker – 14, 21, 31, 45, 66, 97 kDa
- Reduced and boiled sample, 2.5 μg/lane
- Non-reduced and non-boiled sample, 2.5 μg/lane
Endotoxin
< 0.1 EU/μg
Formulation
Filtered (0.4 μm) and lyophilized from 0.5 mg/ml solution in 20 mM Tris buffer, 50 mM NaCl, 1mM CaCl2, pH 7.5.
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely.
Applications
Western blotting, ELISA
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store lyophilized protein at -80°C. Lyophilized protein remains stable until the expiry date when stored at -80°C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at -80°C for long term storage. Reconstituted protein can be stored at 4°C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
Note
This product is intended for research use only.